Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CENP-B CRISPR/Cas9 KO Plasmid (h): sc-401356
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CENP-B CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CENP-B genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CENP-B Antibody (F-4): sc-376283
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CENP-B CRISPR/Cas9 KO Plasmid (h) | sc-401356 | 20 µg | $397.00 |
Overview
CENPB encodes centromere protein B (CENP-B), a sequence-specific DNA-binding factor that recognizes the CENP-B box within α-satellite repeats and helps organize centromeric chromatin. CENP-B contributes to kinetochore assembly and chromosome segregation by supporting centromere architecture and higher-order heterochromatin organization during mitosis. Through its roles in centromere maintenance and cell-cycle progression, altered CENPB function or centromere dysregulation is relevant to genome instability phenotypes, including aneuploidy and chromosomal aberrations observed across diverse cancers. CENP-B is also a well-established autoantigen in anti-centromere antibody responses, linking centromere biology to autoimmune-associated cellular phenotypes.
CENP-B CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CENPB gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CENPB together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CENPB open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CENP-B protein expression.
This CRISPR knockout system enables efficient generation of CENPB-deficient cell models for investigation of CENP-B signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CENPB exon(s) critical for CENP-B function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CENPB genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CENP-B CRISPR/Cas9 KO Plasmid (h) and CENP-B CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CENPB locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CENP-B HDR Plasmid (h) and CENP-B HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CENPB homology arms to support homology-directed repair at defined CENPB target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.