CEACAM5 Antibody (CI-P83-1): sc-23928

CEACAM5 Antibody (CI-P83-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects CEACAM5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Anti-CEACAM5 antibody (CI-P83-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CEACAM5, also known as CD66e, is a heavily glycosylated glycosylphosphatidylinositol (GPI)-anchored protein that plays a crucial role in cell adhesion and signaling, particularly in tumor biology. CEACAM5 expression is significantly upregulated in various cancers, making CEACAM5 a valuable tumor marker for diagnostic and therapeutic applications. CEACAM5 is primarily located on the surface of epithelial cells, where CEACAM5 facilitates intercellular adhesion and communication, essential for maintaining tissue integrity and regulating immune responses. CEACAM5 presence on tumor cells can promote tumor progression and metastasis, highlighting CEACAM5′s importance as a target for cancer immunotherapy. With diverse applications and critical role in cancer biology, CEACAM5 monoclonal antibody (CI-P83-1) serves as an essential tool for researchers studying tumor markers and cell adhesion mechanisms.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CEACAM5 Antibody (CI-P83-1) References:

1. Mutations in the carcinoembryonic antigen gene in colorectal cancer patients: implications on liver metastasis.  |  Zimmer, R. and Thomas, P. 2001. Cancer Res. 61: 2822-6. PMID: 11306451
2. Assembly and analysis of cosmid contigs in the CEA-gene family region of human chromosome 19.  |  Tynan, K., et al. 1992. Nucleic Acids Res. 20: 1629-36. PMID: 1579453
3. Cloning of the complete gene for carcinoembryonic antigen: analysis of its promoter indicates a region conveying cell type-specific expression.  |  Schrewe, H., et al. 1990. Mol Cell Biol. 10: 2738-48. PMID: 2342461
4. Carcinoembryonic antigens: alternative splicing accounts for the multiple mRNAs that code for novel members of the carcinoembryonic antigen family.  |  Barnett, TR., et al. 1989. J Cell Biol. 108: 267-76. PMID: 2537311
5. Isolation and characterization of cDNA clones encoding the human carcinoembryonic antigen reveal a highly conserved repeating structure.  |  Zimmermann, W., et al. 1987. Proc Natl Acad Sci U S A. 84: 2960-4. PMID: 3033671
6. Carcinoembryonic antigen family: characterization of cDNAs coding for NCA and CEA and suggestion of nonrandom sequence variation in their conserved loop-domains.  |  Barnett, T., et al. 1988. Genomics. 3: 59-66. PMID: 3220478
7. Preclinical Activity of SAR408701: A Novel Anti-CEACAM5-maytansinoid Antibody-drug Conjugate for the Treatment of CEACAM5-positive Epithelial Tumors.  |  Decary, S., et al. 2020. Clin Cancer Res. 26: 6589-6599. PMID: 33046521
8. Safety, pharmacokinetics, and antitumor activity of the anti-CEACAM5-DM4 antibody-drug conjugate tusamitamab ravtansine (SAR408701) in patients with advanced solid tumors: first-in-human dose-escalation study.  |  Gazzah, A., et al. 2022. Ann Oncol. 33: 416-425. PMID: 35026412
9. The search for therapeutic targets in lung cancer: Preclinical and human studies of carcinoembryonic antigen-related cell adhesion molecule 5 expression and its associated molecular landscape.  |  Lefebvre, AM., et al. 2023. Lung Cancer. 184: 107356. PMID: 37660479
10. Hypoxia exosome derived CEACAM5 promotes tumor-associated macrophages M2 polarization to accelerate pancreatic neuroendocrine tumors metastasis via MMP9.  |  Ye, M., et al. 2024. FASEB J. 38: e23762. PMID: 38923643
11. CD66 family members are associated with tyrosine kinase activity in human neutrophils.  |  Skubitz, KM., et al. 1995. J Immunol. 155: 5382-90. PMID: 7594554
12. Human biliary glycoprotein gene: characterization of a family of novel alternatively spliced RNAs and their expressed proteins.  |  Barnett, TR., et al. 1993. Mol Cell Biol. 13: 1273-82. PMID: 8423792

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CEACAM5 Antibody (CI-P83-1) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-CEACAM5 Antibody (CI-P83-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CEACAM5 Antibody (CI-P83-1) Specifications

Alternate Product Name: CEACAM5

Product Clone Name: CI-P83-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: CEA-180

Epitope Species: human

Product Reactivity/Detection: CEACAM5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for CEACAM5 Antibody (CI-P83-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin