Cdx1 Antibody (D-4): sc-515146

Cdx1 Antibody (D-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects Cdx1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Cdx1 (D-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cdx1, a member of the caudal-type homeobox gene family, plays a crucial role in the development and differentiation of the intestinal epithelium. Specifically, Cdx1 maintains the intestinal phenotype and regulates cellular growth and differentiation within the intestinal tract. Cdx1 is expressed in both fetal and adult small intestine and colon, and Cdx1 expression is vital for proper anterior-posterior patterning along the crypt-villus axis. Notably, the balance of Cdx1 and Cdx2 expression is significant, as a decrease in either protein is linked to colorectal tumorigenesis, highlighting their roles in tumor suppression and cellular apoptosis. The human Cdx1 gene is located on chromosome 5q31-q33 and encodes a 265-amino acid protein, underscoring its importance in intestinal health and disease.

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Cdx1 Antibody (D-4) References:

1. Cdx1 and cdx2 expression during intestinal development.  |  Silberg, DG., et al. 2000. Gastroenterology. 119: 961-71. PMID: 11040183
2. Cdx1 promotes cellular growth of epithelial intestinal cells through induction of the secretory protein PAP I.  |  Moucadel, V., et al. 2001. Eur J Cell Biol. 80: 156-63. PMID: 11302520
3. Homeobox gene Cdx1 regulates Ras, Rho and PI3 kinase pathways leading to transformation and tumorigenesis of intestinal epithelial cells.  |  Soubeyran, P., et al. 2001. Oncogene. 20: 4180-7. PMID: 11464284
4. The caudal-related homeodomain protein CDX1 activates proliferating cell nuclear antigen expression in hepatocellular and colorectal carcinoma cells.  |  Oh, EJ., et al. 2002. Int J Oncol. 20: 23-9. PMID: 11743638
5. Multiple pathways governing Cdx1 expression during murine development.  |  Prinos, P., et al. 2001. Dev Biol. 239: 257-69. PMID: 11784033
6. RARgamma and Cdx1 interactions in vertebral patterning.  |  Allan, D., et al. 2001. Dev Biol. 240: 46-60. PMID: 11784046
7. The Cdx1 homeodomain protein: an integrator of posterior signaling in the mouse.  |  Lohnes, D. 2003. Bioessays. 25: 971-80. PMID: 14505364
8. CDX1 and CDX2 expression in intestinal metaplasia, dysplasia and gastric cancer.  |  Kang, JM., et al. 2011. J Korean Med Sci. 26: 647-53. PMID: 21532856
9. Loss of the epithelial marker CDX1 predicts poor prognosis in early-stage CRC patients.  |  Solé, L., et al. 2024. Biochim Biophys Acta Mol Cell Res. 1871: 119658. PMID: 38216091
10. Isolation, characterization, and precise physical localization of human CDX1, a caudal-type homeobox gene.  |  Bonner, CA., et al. 1995. Genomics. 28: 206-11. PMID: 8530027
11. Molecular cloning, sequencing and expression of the mRNA encoding human Cdx1 and Cdx2 homeobox. Down-regulation of Cdx1 and Cdx2 mRNA expression during colorectal carcinogenesis.  |  Mallo, GV., et al. 1997. Int J Cancer. 74: 35-44. PMID: 9036867
12. Expression of the Cdx1 and Cdx2 homeotic genes leads to reduced malignancy in colon cancer-derived cells.  |  Mallo, GV., et al. 1998. J Biol Chem. 273: 14030-6. PMID: 9593754

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cdx1 Antibody (D-4) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Cdx1 Antibody (D-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cdx1 Antibody (D-4) Specifications

Alternate Product Name: Cdx1

Product Clone Name: D-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 220-265

Epitope Target: Cdx1

Epitope Species: human

Product Reactivity/Detection: Cdx1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Cdx1 Antibody (D-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin