Cdt2 Antibody (B-8): sc-166735

Cdt2 Antibody (B-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Cdt2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Cdt2 antibody (B-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cdt2, also known as DTL (denticleless homolog), CDW1, DCAF2, L2DTL, or RAMP, is a 730 amino acid protein that plays a crucial role in regulating cell cycle and DNA replication. Cdt2 is primarily localized to the cytoplasm and nuclear membrane, where Cdt2 participates in the proteolysis of Cdt1, a key regulator of DNA replication, particularly in response to DNA damage. The presence of seven WD repeats in Cdt2 facilitates protein-protein interactions and is essential for regulatory functions. Cdt2 is expressed in various tissues, including testis, placenta, bone marrow, thymus, and skeletal muscle, highlighting importance in cellular processes across different biological contexts. Upon DNA damage, Cdt2 undergoes phosphorylation, likely mediated by ATM or ATR kinases, which further underscores a role in maintaining genomic stability. Two isoforms of Cdt2 arise from alternative splicing, adding another layer of complexity to function and regulation.

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Cdt2 Antibody (B-8) References:

1. Cloning and expression of a novel nuclear matrix-associated protein that is regulated during the retinoic acid-induced neuronal differentiation.  |  Cheung, WM., et al. 2001. J Biol Chem. 276: 17083-91. PMID: 11278750
2. L2DTL/CDT2 and PCNA interact with p53 and regulate p53 polyubiquitination and protein stability through MDM2 and CUL4A/DDB1 complexes.  |  Banks, D., et al. 2006. Cell Cycle. 5: 1719-29. PMID: 16861890
3. A family of diverse Cul4-Ddb1-interacting proteins includes Cdt2, which is required for S phase destruction of the replication factor Cdt1.  |  Jin, J., et al. 2006. Mol Cell. 23: 709-21. PMID: 16949367
4. CUL4-DDB1 ubiquitin ligase interacts with multiple WD40-repeat proteins and regulates histone methylation.  |  Higa, LA., et al. 2006. Nat Cell Biol. 8: 1277-83. PMID: 17041588
5. DTL/CDT2 is essential for both CDT1 regulation and the early G2/M checkpoint.  |  Sansam, CL., et al. 2006. Genes Dev. 20: 3117-29. PMID: 17085480
6. Role of L2DTL, cell cycle-regulated nuclear and centrosome protein, in aggressive hepatocellular carcinoma.  |  Pan, HW., et al. 2006. Cell Cycle. 5: 2676-87. PMID: 17106265
7. CDK inhibitor p21 is degraded by a proliferating cell nuclear antigen-coupled Cul4-DDB1Cdt2 pathway during S phase and after UV irradiation.  |  Nishitani, H., et al. 2008. J Biol Chem. 283: 29045-52. PMID: 18703516

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cdt2 Antibody (B-8) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Cdt2 Antibody (B-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cdt2 Antibody (B-8) Specifications

Alternate Product Name: Cdt2

Product Clone Name: B-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-240

Epitope Target: Cdt2

Epitope Species: human

Product Reactivity/Detection: Cdt2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Cdt2 Antibody (B-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin