Cdt1 Antibody (F-6): sc-365305

Cdt1 Antibody (F-6) is a mouse monoclonal IgG2b kappa light chain antibody that detects Cdt1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Cdt1 antibody (F-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cdt1 functions as a nuclear-localizing replication initiation factor, specifically expressed during the G1 and S phases of the cell cycle. Cdt1 facilitates the loading of the minichromosome maintenance (MCM) complex onto chromatin at the end of mitosis, a critical step necessary for DNA replication initiation. While Cdt1 mRNA maintains consistent expression throughout the cell cycle, Cdt1 protein levels decrease significantly after S-phase onset, indicating proteolytic degradation′s essential role in maintaining proper levels and preventing untimely DNA replication. Cdt1 also interacts with DNA replication inhibitor geminin during S and G2 phases, preventing inappropriate origin firing. Cdt1′s ability to reverse geminin′s inhibition of DNA replication in cell-free extracts highlights Cdt1′s significance in DNA synthesis and cell cycle progression regulation.

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Cdt1 Antibody (F-6) References:

1. XCDT1 is required for the assembly of pre-replicative complexes in Xenopus laevis.  |  Maiorano, D., et al. 2000. Nature. 404: 622-5. PMID: 10766247
2. The Cdt1 protein is required to license DNA for replication in fission yeast.  |  Nishitani, H., et al. 2000. Nature. 404: 625-8. PMID: 10766248
3. Inhibition of eukaryotic DNA replication by geminin binding to Cdt1.  |  Wohlschlegel, JA., et al. 2000. Science. 290: 2309-12. PMID: 11125146
4. The human licensing factor for DNA replication Cdt1 accumulates in G1 and is destabilized after initiation of S-phase.  |  Nishitani, H., et al. 2001. J Biol Chem. 276: 44905-11. PMID: 11555648
5. Redundant control of rereplication in fission yeast.  |  Gopalakrishnan, V., et al. 2001. Proc Natl Acad Sci U S A. 98: 13114-9. PMID: 11606752
6. Oncogenic potential of the DNA replication licensing protein CDT1.  |  Arentson, E., et al. 2002. Oncogene. 21: 1150-8. PMID: 11850834
7. Licensing regulators Geminin and Cdt1 identify progenitor cells of the mouse CNS in a specific phase of the cell cycle.  |  Spella, M., et al. 2007. Neuroscience. 147: 373-87. PMID: 17533120
8. Cdt1 degradation to prevent DNA re-replication: conserved and non-conserved pathways.  |  Kim, Y. and Kipreos, ET. 2007. Cell Div. 2: 18. PMID: 17565698
9. Cdt1 overexpression drives colorectal carcinogenesis through origin overlicensing and DNA damage.  |  Petropoulos, M., et al. 2023. J Pathol. 259: 10-20. PMID: 36210634
10. Identification of CDT1 as a prognostic marker in human lung adenocarcinoma using bioinformatics approaches.  |  Jiang, J., et al. 2023. PeerJ. 11: e16166. PMID: 37790630
11. The significance of CDT1 expression in non-cancerous and cancerous liver in cases with hepatocellular carcinoma.  |  Ogawa, M., et al. 2023. J Clin Biochem Nutr. 73: 234-248. PMID: 37970553
12. cdt1 is an essential target of the Cdc10/Sct1 transcription factor: requirement for DNA replication and inhibition of mitosis.  |  Hofmann, JF. and Beach, D. 1994. EMBO J. 13: 425-34. PMID: 8313888

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cdt1 Antibody (F-6) has 22 citations in a variety of scientific publications.

Primary antibodies like Anti-Cdt1 Antibody (F-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cdt1 Antibody (F-6) Specifications

Alternate Product Name: Cdt1

Product Clone Name: F-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 247-546

Epitope Target: Cdt1

Epitope Species: human

Product Reactivity/Detection: Cdt1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 22 citations in various scientific publications

Available Conjugates/Forms for Cdt1 Antibody (F-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin