Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CDKN2C/p18 INK4c CRISPR/Cas9 KO Plasmid (h): sc-401214
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CDKN2C/p18 INK4c CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CDKN2C/p18 INK4c genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CDKN2C/p18 INK4c Antibody (118.2): sc-9965
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CDKN2C/p18 INK4c CRISPR/Cas9 KO Plasmid (h) | sc-401214 | 20 µg | $397.00 |
Overview
CDKN2C encodes p18 INK4c, a member of the INK4 family of cyclin-dependent kinase inhibitors that binds CDK4 and CDK6 to restrain cyclin D–driven phosphorylation of RB and enforce the G1/S checkpoint. By limiting E2F-dependent transcriptional programs, CDKN2C contributes to control of cell-cycle progression, lineage commitment, and cellular quiescence across multiple tissues. Perturbation of CDKN2C function is frequently studied in the context of dysregulated CDK4/6–RB signaling, genomic instability, and proliferative phenotypes relevant to cancer biology. CDKN2C is also used as a mechanistic node to connect mitogenic signaling inputs with checkpoint integrity in models of oncogenic stress.
CDKN2C/p18 INK4c CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CDKN2C gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CDKN2C together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CDKN2C open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CDKN2C/p18 INK4c protein expression.
This CRISPR knockout system enables efficient generation of CDKN2C-deficient cell models for investigation of CDKN2C/p18 INK4c signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CDKN2C exon(s) critical for CDKN2C/p18 INK4c function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CDKN2C genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CDKN2C/p18 INK4c CRISPR/Cas9 KO Plasmid (h) and CDKN2C/p18 INK4c CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CDKN2C locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CDKN2C/p18 INK4c HDR Plasmid (h) and CDKN2C/p18 INK4c HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CDKN2C homology arms to support homology-directed repair at defined CDKN2C target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.