Cdk8 Antibody (D-9): sc-13155

Cdk8 Antibody (D-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects Cdk8 protein of mouse, rat, and human origin through applications such as western blotting, immunoprecipitation, immunofluorescence, immunohistochemistry, and enzyme-linked immunosorbent assay. Cdk8 (D-9) antibody is offered in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. Cdk8 plays a crucial role in regulating cell cycle progression, functioning as part of a larger family of cyclin-dependent kinases (Cdks) that work in tandem with cyclins to phosphorylate essential substrates at different phases of the cell cycle. This regulation is vital for maintaining proper cellular function and ensuring that cells divide accurately and at the right time. Cdk8 forms large complexes with cyclin C and the large subunit of RNA polymerase II, suggesting involvement in transcriptional regulation. By modulating RNA polymerase II activity, Cdk8 influences gene expression, which is critical for various cellular processes, including growth and differentiation. Cdk8 acts as a transcriptional activator when fused to the DNA binding domain of GAL4, highlighting importance in transcriptional control.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Cdk8 Antibody (D-9) References:

1. PCTAIRE-1 and PCTAIRE-3, two members of a novel cdc2/CDC28-related protein kinase gene family.  |  Okuda, T., et al. 1992. Oncogene. 7: 2249-58. PMID: 1437147
2. Conversion of antigen-specific effector/memory T cells into Foxp3-expressing Treg cells by inhibition of CDK8/19.  |  Akamatsu, M., et al. 2019. Sci Immunol. 4: PMID: 31653719
3. CDK8 and CDK19: positive regulators of signal-induced transcription and negative regulators of Mediator complex proteins.  |  Chen, M., et al. 2023. Nucleic Acids Res. 51: 7288-7313. PMID: 37378433
4. CDK8 inhibitors antagonize HIV-1 reactivation and promote provirus latency in T cells.  |  Horvath, RM., et al. 2023. J Virol. 97: e0092323. PMID: 37671866
5. Identification of human cyclin-dependent kinase 8, a putative protein kinase partner for cyclin C.  |  Tassan, JP., et al. 1995. Proc Natl Acad Sci U S A. 92: 8871-5. PMID: 7568034
6. The cell cycle kinases.  |  Pines, J. 1994. Semin Cancer Biol. 5: 305-13. PMID: 7803767
7. Cyclin C/CDK8 is a novel CTD kinase associated with RNA polymerase II.  |  Rickert, P., et al. 1996. Oncogene. 12: 2631-40. PMID: 8700522
8. Drosophila Cdk8, a kinase partner of cyclin C that interacts with the large subunit of RNA polymerase II.  |  Leclerc, V., et al. 1996. Mol Biol Cell. 7: 505-13. PMID: 8730095
9. Cyclins, cyclin-dependent kinases and cdk inhibitors: implications in cell cycle control and cancer.  |  MacLachlan, TK., et al. 1995. Crit Rev Eukaryot Gene Expr. 5: 127-56. PMID: 8845581
10. Viral transactivators E1A and VP16 interact with a large complex that is associated with CTD kinase activity and contains CDK8.  |  Gold, MO., et al. 1996. Nucleic Acids Res. 24: 3771-7. PMID: 8871557
11. Role of the cyclin-dependent kinase 4 and 6 inhibitor gene family p15, p16, p18 and p19 in leukemia and lymphoma.  |  Siebert, R., et al. 1996. Leuk Lymphoma. 23: 505-20. PMID: 9031081
12. Current concepts in neuro-oncology: the cell cycle--a review.  |  Dirks, PB. and Rutka, JT. 1997. Neurosurgery. 40: 1000-13; discussion 1013-5. PMID: 9149259

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cdk8 Antibody (D-9) has 22 citations in a variety of scientific publications.

Primary antibodies like Anti-Cdk8 Antibody (D-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cdk8 Antibody (D-9) Specifications

Alternate Product Name: Cdk8

Product Clone Name: D-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 326-464

Epitope Target: Cdk8

Epitope Species: human

Product Reactivity/Detection: Cdk8

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 22 citations in various scientific publications

Available Conjugates/Forms for Cdk8 Antibody (D-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, TRITC: Rhodamine