Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Cdk6 CRISPR/Cas9 KO Plasmid (h): sc-400309
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cdk6 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Cdk6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Cdk6 Antibody (B-10): sc-7961
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdk6 CRISPR/Cas9 KO Plasmid (h) | sc-400309 | 20 µg | $397.00 |
Overview
CDK6 encodes cyclin-dependent kinase 6 (Cdk6), a serine/threonine kinase that partners with D-type cyclins to phosphorylate RB family proteins and promote G1-to-S phase cell-cycle progression. Cdk6 activity integrates mitogenic signaling with checkpoint control through the cyclin D–CDK4/6–RB–E2F axis and interfaces with pathways governing proliferation, differentiation, and senescence. Dysregulated CDK6 expression or signaling is frequently associated with aberrant cell-cycle control in cancer and is also implicated in hematopoietic lineage decisions and inflammatory transcriptional programs. Because of its central role in coordinating cell-cycle entry, CDK6 is widely studied in tumor biology, stem/progenitor cell regulation, and mechanisms of therapy resistance at the pathway level.
Cdk6 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CDK6 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CDK6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CDK6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Cdk6 protein expression.
This CRISPR knockout system enables efficient generation of CDK6-deficient cell models for investigation of Cdk6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CDK6 exon(s) critical for Cdk6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CDK6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Cdk6 CRISPR/Cas9 KO Plasmid (h) and Cdk6 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CDK6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Cdk6 HDR Plasmid (h) and Cdk6 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CDK6 homology arms to support homology-directed repair at defined CDK6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.