Cdk4 Antibody (F-8): sc-393653

Cdk4 Antibody (F-8) is a mouse monoclonal IgG1 kappa light chain antibody developed to detect Cyclin-dependent kinase 4 (Cdk4), also known as CDC2-like kinase 4, in mouse, rat, and human samples. anti-Cdk4 antibody (F-8) recognizes an epitope mapping between amino acids 278-303 at the C-terminus of the full-length recombinant mouse Cdk4 protein, making Cdk4 (F-8) antibody ideal for applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Cdk4 is a pivotal serine/threonine kinase involved in regulating the cell cycle, particularly the transition from the G1 phase to the S phase, by forming complexes with D-type cyclins. Post-translational modifications of Cdk4, especially phosphorylation, are critical for activation and regulation, enabling precise control over cell cycle progression and preventing uncontrolled cell proliferation that can lead to cancer. The phosphorylation of Cdk4 by Cdk-activating kinases (CAKs) enhances kinase activity, facilitating the phosphorylation of key substrates such as the retinoblastoma protein (Rb), which is essential for the release of E2F transcription factors and subsequent DNA synthesis. Additionally, Cdk4 interacts with regulatory proteins like the inhibitor p16, which modulates activity to maintain proper cell cycle regulation and prevent tumorigenesis. Available in both non-conjugated and various conjugated forms, including horseradish peroxidase (HRP) and fluorescent tags, Cdk4 monoclonal antibody (F-8) offers versatility to meet diverse research needs. By facilitating the detailed analysis of Cdk4′s function and regulation, Cdk4 (F-8) monoclonal antibody supports advancements in cancer research and the development of targeted therapeutic strategies.

The preferred Cdk4 antibody is Cdk4 Antibody (DCS-35): sc-23896.