Cdk4 Antibody (B-10): sc-166373

Cdk4 Antibody (B-10) is a mouse monoclonal IgG1 kappa light chain antibody designed to detect Cyclin-dependent kinase 4 (Cdk4), also known as CDC2-like kinase 4, in mouse, rat, and human samples. anti-Cdk4 antibody (B-10) targets an epitope encompassing amino acids 1-303 of the full-length recombinant human Cdk4 protein, making Cdk4 (B-10) antibody ideal for applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Cdk4 is a critical serine/threonine kinase located primarily in the nucleus and cytoplasm, where Cdk4 forms complexes with D-type cyclins to regulate the progression of the cell cycle from the G1 to the S phase. The precise cellular localization of Cdk4 is essential for function, as Cdk4 must be present in the nucleus to phosphorylate key substrates like the retinoblastoma protein (Rb), facilitating the release of E2F transcription factors that drive DNA synthesis and cell division. Proper localization ensures that Cdk4 effectively controls cell proliferation, while mislocalization can lead to uncontrolled cell growth, a characteristic of cancer. Additionally, the dynamic distribution of Cdk4 within the cell allows for responses to various regulatory signals, maintaining cellular homeostasis and ensuring accurate cell cycle progression. Available in both non-conjugated and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, Cdk4 monoclonal antibody (B-10) offers versatile options to meet diverse experimental needs.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cdk4 Antibody (B-10) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-Cdk4 Antibody (B-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cdk4 Antibody (B-10) Specifications

Alternate Product Name: Cdk4

Product Clone Name: B-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-303

Epitope Target: Cdk4

Epitope Species: human

Product Reactivity/Detection: Cdk4 p34

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for Cdk4 Antibody (B-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Cdk4 antibody is Cdk4 Antibody (DCS-35): sc-23896.