CdcA1 Antibody (E-6): sc-271251
- CdcA1 Antibody (E-6) is a mouse monoclonal IgG1 κ CdcA1 antibody, cited in 8 publications, provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of CdcA1 of human origin
- CdcA1 Antibody (E-6) is recommended for detection of CdcA1 of human origin by WB, IP, IF and ELISA
- Anti-CdcA1 Antibody (E-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CdcA1 Antibody (E-6) for WB applications. These reagents are now offered in bundles with CdcA1 Antibody (E-6) (see ordering information below).
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CdcA1 Antibody (E-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects CdcA1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CdcA1 (E-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CdcA1, also known as Cell Division Associated 1, plays a critical role in regulating cell-cycle progression as a member of the centromere protein complex, which is essential for proper chromosome segregation during cell division. Specifically, CdcA1 interacts with kinetochore associated 2 (KNTC2), and together they form a complex vital for dynamic connections between kinetochores and spindle microtubules. This interaction is particularly important in non-small cell lung carcinomas (NSCLC), where both CdcA1 and KNTC2 have been implicated in tumorigenesis. Targeting the CdcA1-KNTC2 complex or selectively inhibiting their activities presents a promising therapeutic strategy for lung cancer treatment, highlighting CdcA1′s significance in both cell biology and cancer research.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CdcA1 Antibody (E-6) References:
- A conserved protein, Nuf2, is implicated in connecting the centromere to the spindle during chromosome segregation: a link between the kinetochore function and the spindle checkpoint. | Nabetani, A., et al. 2001. Chromosoma. 110: 322-34. PMID: 11685532
- Drug target discovery by gene expression analysis: cell cycle genes. | Walker, MG. 2001. Curr Cancer Drug Targets. 1: 73-83. PMID: 12188893
- Dynamic behavior of Nuf2-Hec1 complex that localizes to the centrosome and centromere and is essential for mitotic progression in vertebrate cells. | Hori, T., et al. 2003. J Cell Sci. 116: 3347-62. PMID: 12829748
- Identification of the substrates and interaction proteins of aurora kinases from a protein-protein interaction model. | Tien, AC., et al. 2004. Mol Cell Proteomics. 3: 93-104. PMID: 14602875
- Nuf2 and Hec1 are required for retention of the checkpoint proteins Mad1 and Mad2 to kinetochores. | DeLuca, JG., et al. 2003. Curr Biol. 13: 2103-9. PMID: 14654001
- Kinetochore localization and microtubule interaction of the human spindle checkpoint kinase Mps1. | Stucke, VM., et al. 2004. Chromosoma. 113: 1-15. PMID: 15235793
- Timing and checkpoints in the regulation of mitotic progression. | Meraldi, P., et al. 2004. Dev Cell. 7: 45-60. PMID: 15239953
- Architecture of the human ndc80-hec1 complex, a critical constituent of the outer kinetochore. | Ciferri, C., et al. 2005. J Biol Chem. 280: 29088-95. PMID: 15961401
- Activation of CDCA1-KNTC2, members of centromere protein complex, involved in pulmonary carcinogenesis. | Hayama, S., et al. 2006. Cancer Res. 66: 10339-48. PMID: 17079454
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CdcA1 Antibody (E-6) | sc-271251 | 200 µg/ml | $322.00 | |||
CdcA1 Antibody (E-6): m-IgG Fc BP-HRP Bundle | sc-529149 | 200 µg Ab; 10 µg BP | $361.00 | |||
CdcA1 Antibody (E-6): m-IgGκ BP-HRP Bundle | sc-521892 | 200 µg Ab, 40 µg BP | $361.00 | |||
CdcA1 Antibody (E-6): m-IgG1 BP-HRP Bundle | sc-543354 | 200 µg Ab; 20 µg BP | $361.00 | |||
CdcA1 Antibody (E-6) AC | sc-271251 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CdcA1 Antibody (E-6) HRP | sc-271251 HRP | 200 µg/ml | $322.00 | |||
CdcA1 Antibody (E-6) FITC | sc-271251 FITC | 200 µg/ml | $336.00 | |||
CdcA1 Antibody (E-6) PE | sc-271251 PE | 200 µg/ml | $349.00 | |||
CdcA1 Antibody (E-6) Alexa Fluor® 488 | sc-271251 AF488 | 200 µg/ml | $364.00 | |||
CdcA1 Antibody (E-6) Alexa Fluor® 546 | sc-271251 AF546 | 200 µg/ml | $364.00 | |||
CdcA1 Antibody (E-6) Alexa Fluor® 594 | sc-271251 AF594 | 200 µg/ml | $364.00 | |||
CdcA1 Antibody (E-6) Alexa Fluor® 647 | sc-271251 AF647 | 200 µg/ml | $364.00 | |||
CdcA1 Antibody (E-6) Alexa Fluor® 680 | sc-271251 AF680 | 200 µg/ml | $364.00 | |||
CdcA1 Antibody (E-6) Alexa Fluor® 790 | sc-271251 AF790 | 200 µg/ml | $364.00 |