Anti-Cdc28 Antibody (G-7) recommended for detection of Cdc28 of y origin by WB, IP, IF and ELISA

Anti-Cdc28 Antibody (G-7): sc-515762

Anti-Cdc28 Antibody (G-7) is rated 5.0 out of 5 by 1.
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Datasheets

    • Anti-Cdc28 Antibody (G-7) is a mouse monoclonal IgG1 (kappa light chain) Cdc28 antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 241-259 near the C-terminus of Cdc28 of Saccharomyces cerevisiae origin
    • Cdc28 Antibody (G-7) is recommended for detection of Cdc28 of y origin by WB, IP, IF and ELISA
    • Anti-Cdc28 Antibody (G-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cdc28 (G-7): sc-515762.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cdc28 Antibody (G-7) for WB applications. These reagents are now offered in bundles with Cdc28 Antibody (G-7) (see ordering information below).
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What is the recommended starting dilution for Western blot for Cdc28 (G-7): sc-515762 monoclonal antibody?

Asked by: Randy McDonald
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-515762.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from good antibody for western blotting this antibody is good for Cdc28 total protein level detection by WB (1:2000 dilution)
Date published: 2018-04-27
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