Cdc28 Antibody (G-7): sc-515762
- Cdc28 Antibody (G-7) is a mouse monoclonal IgG1 κ Cdc28 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 241-259 near the C-terminus of Cdc28 of Saccharomyces cerevisiae origin
- Cdc28 Antibody (G-7) is recommended for detection of Cdc28 of y origin by WB, IP, IF and ELISA
- Anti-Cdc28 Antibody (G-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cdc28 Antibody (G-7) for WB applications. These reagents are now offered in bundles with Cdc28 Antibody (G-7) (see ordering information below).
Cdc28 Antibody (G-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects Cdc28 protein of Saccharomyces cerevisiae origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Cdc28 antibody (G-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cdc28 protein plays a crucial role in regulating the cell cycle, particularly at the G1 phase transition known as Start, which is essential for proper cell division and growth. Cdc28 activity is tightly regulated through association with G1 cyclins such as Cln1, Cln2, and Cln3, which are necessary for the transition from G1 to S phase. Cdc28 interactions with M phase cyclins, including Clb1 and Clb2, are vital for the progression from G2 to M phase, while S phase cyclins Clb5 and Clb6 ensure that DNA replication is coordinated with cytokinesis. Expression of these cyclins is regulated by ubiquitin-mediated proteolysis involving Ubc9 and Cdc34, highlighting Cdc28′s importance in maintaining cell cycle integrity.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Cdc28 Antibody (G-7) References:
- Negative regulation of G1 and G2 by S-phase cyclins of Saccharomyces cerevisiae. | Basco, RD., et al. 1995. Mol Cell Biol. 15: 5030-42. PMID: 7651421
- Increased ubiquitin expression suppresses the cell cycle defect associated with the yeast ubiquitin conjugating enzyme, CDC34 (UBC3). Evidence for a noncovalent interaction between CDC34 and ubiquitin. | Prendergast, JA., et al. 1995. J Biol Chem. 270: 9347-52. PMID: 7721857
- Role of a ubiquitin-conjugating enzyme in degradation of S- and M-phase cyclins. | Seufert, W., et al. 1995. Nature. 373: 78-81. PMID: 7800043
- Starting to cycle: G1 controls regulating cell division in budding yeast. | Sherlock, G. and Rosamond, J. 1993. J Gen Microbiol. 139: 2531-41. PMID: 8277239
- Mechanisms that help the yeast cell cycle clock tick: G2 cyclins transcriptionally activate G2 cyclins and repress G1 cyclins. | Amon, A., et al. 1993. Cell. 74: 993-1007. PMID: 8402888
- Control of the yeast cell cycle by the Cdc28 protein kinase. | Nasmyth, K. 1993. Curr Opin Cell Biol. 5: 166-79. PMID: 8507488
- G2 cyclins are required for the degradation of G1 cyclins in yeast. | Blondel, M. and Mann, C. 1996. Nature. 384: 279-82. PMID: 8918881
- Saccharomyces cerevisiae G1 cyclins differ in their intrinsic functional specificities. | Levine, K., et al. 1996. Mol Cell Biol. 16: 6794-803. PMID: 8943334
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdc28 Antibody (G-7) | sc-515762 | 200 µg/ml | $322.00 | |||
Cdc28 Antibody (G-7): m-IgG Fc BP-HRP Bundle | sc-531505 | 200 µg Ab; 10 µg BP | $361.00 | |||
Cdc28 Antibody (G-7): m-IgGκ BP-HRP Bundle | sc-525205 | 200 µg Ab, 40 µg BP | $361.00 | |||
Cdc28 Antibody (G-7): m-IgG1 BP-HRP Bundle | sc-544597 | 200 µg Ab; 20 µg BP | $361.00 | |||
Cdc28 Antibody (G-7) AC | sc-515762 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Cdc28 Antibody (G-7) HRP | sc-515762 HRP | 200 µg/ml | $322.00 | |||
Cdc28 Antibody (G-7) FITC | sc-515762 FITC | 200 µg/ml | $336.00 | |||
Cdc28 Antibody (G-7) PE | sc-515762 PE | 200 µg/ml | $349.00 | |||
Cdc28 Antibody (G-7) Alexa Fluor® 488 | sc-515762 AF488 | 200 µg/ml | $364.00 | |||
Cdc28 Antibody (G-7) Alexa Fluor® 546 | sc-515762 AF546 | 200 µg/ml | $364.00 | |||
Cdc28 Antibody (G-7) Alexa Fluor® 594 | sc-515762 AF594 | 200 µg/ml | $364.00 | |||
Cdc28 Antibody (G-7) Alexa Fluor® 647 | sc-515762 AF647 | 200 µg/ml | $364.00 | |||
Cdc28 Antibody (G-7) Alexa Fluor® 680 | sc-515762 AF680 | 200 µg/ml | $364.00 | |||
Cdc28 Antibody (G-7) Alexa Fluor® 790 | sc-515762 AF790 | 200 µg/ml | $364.00 |