Cdc25C Antibody (H-6): sc-13138

Cdc25C Antibody (H-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects Cdc25C of human origin through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA). Cdc25C (H-6) antibody is offered in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. Cdc25C plays a crucial role in regulating the cell cycle by acting as a phosphatase that activates cyclin-dependent kinases (Cdks), which are essential for the progression of the cell cycle. Specifically, Cdc25C dephosphorylates and activates Cdk2 and Cdc2, facilitating the transition from the G2 phase to mitosis. This activation is vital for proper cell division and DNA replication, as Cdc25C ensures that cells only enter mitosis when fully prepared, thereby maintaining genomic stability. Dysregulation of Cdc25C can lead to uncontrolled cell proliferation and is implicated in various cancers, making Cdc25C an important target for research and therapeutic interventions.

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Cdc25C Antibody (H-6) References:

1. Inactivation of the p34cdc2-cyclin B complex by the human WEE1 tyrosine kinase.  |  Parker, LL. and Piwnica-Worms, H. 1992. Science. 257: 1955-7. PMID: 1384126
2. cdc25 is a nuclear protein expressed constitutively throughout the cell cycle in nontransformed mammalian cells.  |  Girard, F., et al. 1992. J Cell Biol. 118: 785-94. PMID: 1500423
3. Specific activation of cdc25 tyrosine phosphatases by B-type cyclins: evidence for multiple roles of mitotic cyclins.  |  Galaktionov, K. and Beach, D. 1991. Cell. 67: 1181-94. PMID: 1836978
4. Wee1(+)-like gene in human cells.  |  Igarashi, M., et al. 1991. Nature. 353: 80-3. PMID: 1840647
5. cdc25 is a specific tyrosine phosphatase that directly activates p34cdc2.  |  Gautier, J., et al. 1991. Cell. 67: 197-211. PMID: 1913817
6. Human homolog of fission yeast cdc25 mitotic inducer is predominantly expressed in G2.  |  Sadhu, K., et al. 1990. Proc Natl Acad Sci U S A. 87: 5139-43. PMID: 2195549
7. The role of CDC25C in cell cycle regulation and clinical cancer therapy: a systematic review.  |  Liu, K., et al. 2020. Cancer Cell Int. 20: 213. PMID: 32518522
8. Downregulation of CDC25C in NPCs Disturbed Cortical Neurogenesis.  |  Zhou, X., et al. 2023. Int J Mol Sci. 24: PMID: 36675024
9. PUF60 promotes cell cycle and lung cancer progression by regulating alternative splicing of CDC25C.  |  Xu, N., et al. 2023. Cell Rep. 42: 113041. PMID: 37682709
10. ERβ-activated LINC01018 promotes endometriosis development by regulating the CDC25C/CDK1/CyclinB1 pathway.  |  Lu, R., et al. 2024. J Genet Genomics. 51: 617-629. PMID: 38224945
11. Negative regulation of the wee1 protein kinase by direct action of the nim1/cdr1 mitotic inducer.  |  Coleman, TR., et al. 1993. Cell. 72: 919-29. PMID: 7681363
12. Phosphorylation and inactivation of the mitotic inhibitor Wee1 by the nim1/cdr1 kinase.  |  Parker, LL., et al. 1993. Nature. 363: 736-8. PMID: 8515817

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cdc25C Antibody (H-6) has 88 citations in a variety of scientific publications.

Primary antibodies like Anti-Cdc25C Antibody (H-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cdc25C Antibody (H-6) Specifications

Alternate Product Name: Cdc25C

Product Clone Name: H-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-150

Epitope Target: Cdc25C

Epitope Species: human

Product Reactivity/Detection: Cdc25C

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 88 citations in various scientific publications

Available Conjugates/Forms for Cdc25C Antibody (H-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, TRITC: Rhodamine