Cdc25A Antibody (DCS-124) recommended for detection of Cdc25A of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Cdc25A Antibody (DCS-124): sc-65505

Cdc25A Antibody (DCS-124) is rated 3.0 out of 5 by 1.
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Datasheets

    • Cdc25A Antibody (DCS-124) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against full length Cdc25A of human origin
    • recommended for detection of Cdc25A of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; detects ~67 kDa and ~60 kDa proteins corresponding to CDC25A and an alternately spliced isoform of CDC25A
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See Cdc25A (F-6): sc-7389 for Cdc25A antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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Can Cdc25A (DCS-124): sc-65505 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Professor Griffin
Thank you for your question. Yes, Cdc25A (DCS-124): sc-65505 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 3 out of 5 by from Clean WB blot with single strong band in Clean WB blot with single strong band in human Cdc25A transfected 293 whole cell lysates. -SCBT QC
Date published: 2014-09-04
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