Cdc25A Antibody (F-6) recommended for detection of Cdc25A of mouse, rat and human origin by WB, IP and IF

Cdc25A Antibody (F-6): sc-7389

Cdc25A Antibody (F-6) is rated 4.1 out of 5 by 16.
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Datasheets

    • Anti-Cdc25A Antibody (F-6) is a mouse monoclonal IgG1 (kappa light chain) Cdc25A antibody provided at 200 µg/ml
    • epitope mapping at the C-terminus of Cdc25A of mouse origin
    • Anti-Cdc25A Antibody (F-6) is recommended for detection of Cdc25A of mouse, rat and human origin by WB, IP and IF
    • Anti-Cdc25A Antibody (F-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cdc25A (F-6): sc-7389.
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How can I get the antigen peptide for Competition Assay to verify the specific bands in my samples?

Asked by: Stav
This antibody is not raised against a peptide, but rather is raised against a recombinant protein. Therefore, no blocking peptide antigen is available. We would recommend using alternative positive and negative controls to verify the specificity of your results with this antibody.
Answered by: Technical Support
Date published: 2019-04-13

I bought this antibody after seeing several citations, however it does not show any bands at 1:200 dilution as suggested by other users. I used U2OS cell lysate known to express CDC25A from many publications. Please help me !

Asked by: emily and jack
Thank you for your question. Please contact our Technical Service Department and we can assist you in troubleshooting the antibody, Cdc25A (F-6): sc-7389. A dilution of 1:200 should be appropriate, and we recommend loading 50 µg of whole cell lysate protein. A link to our complete recommended protocol for Western blotting is below as a reference. Please call our Asian Support office toll-free at 800-820-8626, email [email protected], or contact us by live chat. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-11-18

I recently brought Cdc25a antibody from Santa Cruz (sc-7389) , after seeing several citations. But I am not getting any expression in HEK293T cells, while several articles showed Cdc25A expression in HEK293T cells. Please suggest me some tips?

Asked by: Das S
We are sorry to hear that this antibody is not performing for you as expected. We want you to be successful in your research. Please contact our Technical Service Department by phone, e-mail or live chat, or reach out to your local distributor. We are happy to help you troubleshoot the antibody!
Answered by: Tech Service
Date published: 2017-06-21

What is the recommended secondary when using this antibody for WB?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: TechService7
Date published: 2016-12-23
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Rated 3 out of 5 by from anti-CDC25A Good monoclonal antibody for the detection of endogenous CDC25A by Western blot. Recommended dilution: 1/100
Date published: 2019-02-11
Rated 3 out of 5 by from Great to detect degradation after damage We've been using this for quite long time. It worked nicely to detect Cdc25A degradation, especially after DNA damage. It worked better for human, for mouse it is much weaker.
Date published: 2018-01-26
Rated 4 out of 5 by from It works well This is a free sample, and I try it in Hela cells, it works well, but may need high dilution (1:100).
Date published: 2018-01-08
Rated 5 out of 5 by from Great features! I bought this a month ago and am so happy that I had gotten a perfect picture of Western blot analysis.
Date published: 2017-12-12
Rated 2 out of 5 by from The function is not good It works in western blot at 1:100,gastric cancers. but we cannot get a clear band.
Date published: 2017-09-21
Rated 5 out of 5 by from GREAT FEATURES I can get clear bands when using protein samples from T24 and 5637 in WB.
Date published: 2017-09-08
Rated 5 out of 5 by from Works Great! I had been trying many other antibodies from different companies for cdc25A and was having no luck. This antibody works great for western blotting at a 1:200 dilution. I highly recommend this antibody!
Date published: 2017-04-27
Rated 5 out of 5 by from Works WB We get pretty results in WB, this antibody has high titer and specificity, with a strong and clean band.
Date published: 2017-03-18
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