Date published: 2026-5-10

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Cdc25A Antibody (3H2016): sc-70823

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Datasheets
  • Cdc25A Antibody (3H2016) is a mouse monoclonal IgG2a κ, cited in 5 publications, provided at 200 µg/ml
  • raised against purified recombinant Cdc25A
  • recommended for detection of Cdc25A of mouse, rat and human origin by WB, IP, IF and IHC(P)
  • See Cdc25A (F-6): sc-7389 for Cdc25A antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cdc25A Antibody (3H2016) for WB and IHC(P) applications. These reagents are now offered in bundles with Cdc25A Antibody (3H2016) (see ordering information below).
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Cdc25A Antibody (3H2016) is a mouse monoclonal IgG2a light chain antibody that detects Cdc25A in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Cdc25A plays a crucial role in cell cycle regulation, particularly in the transition from G2 phase to mitosis, by acting as a phosphatase that dephosphorylates and activates the cyclin-dependent kinase Cdc2. This activation is essential for proper progression of mitosis, as Cdc25A allows cells to enter the mitotic phase at the right time, ensuring accurate cell division and maintaining genomic stability. Dysregulation of Cdc25A can lead to uncontrolled cell proliferation and is implicated in various cancers, making Cdc25A a significant target for therapeutic intervention. Anti-Cdc25A antibody (3H2016) provides reliable detection of Cdc25A, facilitating research into cell cycle dynamics and potential biomarkers for cancer progression.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Cdc25A Antibody (3H2016)

sc-70823
200 µg/ml
$322.00

Cdc25A Antibody (3H2016): m-IgGκ BP-HRP Bundle

sc-534399
200 µg Ab; 40 µg BP
$361.00

Cdc25A Antibody (3H2016): m-IgG2a BP-HRP Bundle

sc-546187
200 µg Ab; 10 µg BP
$361.00

Can Cdc25A (3H2016): sc-70823 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: TinTin
Thank you for your question. Yes, Cdc25A (3H2016): sc-70823 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-28
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Rated 4 out of 5 by from Works well with human Cdc25A transfectedWorks well with human Cdc25A transfected 293 whole cell lysates. -SCBT QC
Date published: 2015-03-03
Rated 4 out of 5 by from Published data in a cohort of 313 patientsPublished data in a cohort of 313 patients using this antibody for Western blot analysis. -SCBT Publication Review
Date published: 2015-01-11
Rated 3 out of 5 by from Strong band with some background for WB inStrong band with some background for WB in HeLa (A) and K-562 (B) whole cell lysates. -SCBT QC
Date published: 2014-11-13
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Cdc25A Antibody (3H2016) is rated 3.7 out of 5 by 3.
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