Cdc25A Antibody (3H2016) recommended for detection of Cdc25A of mouse, rat and human origin by WB, IP, IF and IHC(P)

Cdc25A Antibody (3H2016): sc-70823

Cdc25A Antibody (3H2016) is rated 3.7 out of 5 by 3.
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Datasheets

    • Cdc25A Antibody (3H2016) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against purified recombinant Cdc25A
    • recommended for detection of Cdc25A of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See Cdc25A (F-6): sc-7389 for Cdc25A antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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Can Cdc25A (3H2016): sc-70823 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: TinTin
Thank you for your question. Yes, Cdc25A (3H2016): sc-70823 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-28
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Rated 4 out of 5 by from Works well with human Cdc25A transfected Works well with human Cdc25A transfected 293 whole cell lysates. -SCBT QC
Date published: 2015-03-03
Rated 4 out of 5 by from Published data in a cohort of 313 patients Published data in a cohort of 313 patients using this antibody for Western blot analysis. -SCBT Publication Review
Date published: 2015-01-11
Rated 3 out of 5 by from Strong band with some background for WB in Strong band with some background for WB in HeLa (A) and K-562 (B) whole cell lysates. -SCBT QC
Date published: 2014-11-13
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