Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Cdc20B CRISPR/Cas9 KO Plasmid (h): sc-406774
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cdc20B CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Cdc20B genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Cdc20B Antibody (2F2): sc-517066
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdc20B CRISPR/Cas9 KO Plasmid (h) | sc-406774 | 20 µg | $397.00 |
Overview
CDC20B (cell division cycle 20B) encodes a Cdc20-like protein predicted to participate in cell-cycle–associated regulation, with sequence similarity to APC/C co-activators that coordinate ubiquitin-mediated proteolysis during mitotic progression. Although less well characterized than CDC20, CDC20B expression and genomic context have been linked to proliferative programs and differentiation states in select tissues, suggesting roles in controlling transitions that couple cell-cycle timing with cellular remodeling. Perturbation of CDC20B may therefore influence pathways governing checkpoint control, chromosomal stability, and regulated protein turnover. These processes are frequently altered in cancer and other disorders featuring dysregulated proliferation, making CDC20B a useful target for mechanistic studies of growth control.
Cdc20B CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CDC20B gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CDC20B together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CDC20B open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Cdc20B protein expression.
This CRISPR knockout system enables efficient generation of CDC20B-deficient cell models for investigation of Cdc20B signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CDC20B exon(s) critical for Cdc20B function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CDC20B genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Cdc20B CRISPR/Cas9 KO Plasmid (h) and Cdc20B CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CDC20B locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Cdc20B HDR Plasmid (h) and Cdc20B HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CDC20B homology arms to support homology-directed repair at defined CDC20B target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.