Cdc20 Antibody (E-7): sc-13162

Cdc20 Antibody (E-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects Cdc20 of mouse, rat, and human origin through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry (IHC). Cdc20 monoclonal antibody (E-7) is offered in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. Cdc20 plays a crucial role in cell cycle regulation, particularly in the transition from the G2 phase to mitosis, by activating the anaphase-promoting complex/cyclosome (APC/C), which is essential for the degradation of cyclins and other regulatory proteins. This degradation is vital for the proper progression of the cell cycle, ensuring that cells divide accurately and at the right time. Dysregulation of Cdc20 can lead to improper cell division and has been implicated in various cancers, making Cdc20 an important target for research in cell cycle dynamics and cancer biology. Cdc20 transcript is readily detectable in various cultured cell lines during the growth phase, but expression diminishes when cell growth is chemically arrested, highlighting Cdc20′s role in cell proliferation.

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Cdc20 Antibody (E-7) References:

1. Human p55(CDC)/Cdc20 associates with cyclin A and is phosphorylated by the cyclin A-Cdk2 complex.  |  Ohtoshi, A., et al. 2000. Biochem Biophys Res Commun. 268: 530-4. PMID: 10679238
2. Oscillation of MPF is accompanied by periodic association between cdc25 and cdc2-cyclin B.  |  Jessus, C. and Beach, D. 1992. Cell. 68: 323-32. PMID: 1310257
3. Regulation of neuronal cdc20 (p55cdc) expression by the plasticity-related transcription factor zif268.  |  Conway, AM., et al. 2007. Synapse. 61: 463-8. PMID: 17372985
4. Reversible tyrosine phosphorylation of cdc2: dephosphorylation accompanies activation during entry into mitosis.  |  Morla, AO., et al. 1989. Cell. 58: 193-203. PMID: 2473839
5. Isolation of a human cyclin cDNA: evidence for cyclin mRNA and protein regulation in the cell cycle and for interaction with p34cdc2.  |  Pines, J. and Hunter, T. 1989. Cell. 58: 833-46. PMID: 2570636
6. cdc2 is a component of the M phase-specific histone H1 kinase: evidence for identity with MPF.  |  Arion, D., et al. 1988. Cell. 55: 371-8. PMID: 2844417
7. The Xenopus cdc2 protein is a component of MPF, a cytoplasmic regulator of mitosis.  |  Dunphy, WG., et al. 1988. Cell. 54: 423-31. PMID: 3293802
8. p13suc1 acts in the fission yeast cell division cycle as a component of the p34cdc2 protein kinase.  |  Brizuela, L., et al. 1987. EMBO J. 6: 3507-14. PMID: 3322810
9. Current Progress and Perspectives of CDC20 in Female Reproductive Cancers.  |  Ni, K. and Hong, L. 2023. Curr Mol Med. 23: 193-199. PMID: 35319365
10. The oncogenic role of EIF4A3/CDC20 axis in the endometrial cancer.  |  Lin, Y., et al. 2024. J Mol Med (Berl). 102: 1395-1410. PMID: 39316093
11. The Tumor Suppressor TPD52-Governed Endoplasmic Reticulum Stress is Modulated by APCCdc20.  |  Dan, W., et al. 2024. Adv Sci (Weinh). 11: e2405441. PMID: 39401430
12. A novel mammalian protein, p55CDC, present in dividing cells is associated with protein kinase activity and has homology to the Saccharomyces cerevisiae cell division cycle proteins Cdc20 and Cdc4.  |  Weinstein, J., et al. 1994. Mol Cell Biol. 14: 3350-63. PMID: 7513050

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cdc20 Antibody (E-7) has 115 citations in a variety of scientific publications.

Primary antibodies like Anti-Cdc20 Antibody (E-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cdc20 Antibody (E-7) Specifications

Alternate Product Name: Cdc20

Product Clone Name: E-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-175

Epitope Target: Cdc20

Epitope Species: human

Product Reactivity/Detection: Cdc20

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 115 citations in various scientific publications

Available Conjugates/Forms for Cdc20 Antibody (E-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, TRITC: Rhodamine