Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Cdc2 p34 CRISPR/Cas9 KO Plasmid (m): sc-419582
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cdc2 p34 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Cdc2 p34 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Cdc2 p34 Antibody (17): sc-54
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdc2 p34 CRISPR/Cas9 KO Plasmid (m) | sc-419582 | 20 µg | $397.00 |
Overview
Cdk1 encodes the cyclin-dependent kinase Cdc2 p34, a core driver of cell-cycle progression that controls entry into mitosis and coordinates checkpoints through phosphorylation of multiple substrates. In complex with cyclins, CDK1 regulates centrosome dynamics, chromosome condensation, nuclear envelope breakdown, and spindle assembly, integrating signals from ATM/ATR-mediated DNA damage responses and replication stress pathways. Because CDK1 activity constrains genome stability and proliferative capacity, altered regulation of the CDK1–cyclin axis is frequently implicated in hyperproliferative phenotypes, aneuploidy, and tumor-associated cell-cycle rewiring. Mouse Cdk1 models are therefore widely used to dissect conserved mechanisms of mitotic control, checkpoint fidelity, and replication-to-mitosis transitions in development and disease-relevant contexts.
Cdc2 p34 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Cdk1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cdk1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cdk1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Cdc2 p34 protein expression.
This CRISPR knockout system enables efficient generation of Cdk1-deficient cell models for investigation of Cdc2 p34 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Cdk1 exon(s) critical for Cdc2 p34 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Cdk1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Cdc2 p34 CRISPR/Cas9 KO Plasmid (m) and Cdc2 p34 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cdk1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Cdc2 p34 HDR Plasmid (m) and Cdc2 p34 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cdk1 homology arms to support homology-directed repair at defined Cdk1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.