Cdc2 p34 Antibody (F-10) recommended for detection of Cdc2 p34 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Cdc2 p34 Antibody (F-10): sc-166135

Cdc2 p34 Antibody (F-10) is rated 5.0 out of 5 by 3.
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Datasheets

    • Cdc2 p34 Antibody (F-10) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-297 representing full length Cdc2 p34 of human origin
    • recommended for detection of Cdc2 p34 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See Cdc2 p34 (17): sc-54 for Cdc2 p34 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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I am using Cdc2 p34 (F-10): sc-166135 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Professor Griffin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Nuclear and cytoplasmic staining of glandular Nuclear and cytoplasmic staining of glandular cells immunoperoxidase staining of formalin fixed, paraffin-embedded human breast tissue -SCBT QC
Date published: 2015-03-16
Rated 5 out of 5 by from Positive control via western blot analysis Positive control via western blot analysis of Cdc2 p34 expression in K-562 (A), A-431 (B), HL-60 (C) and HeLa (D) whole cell lysates. -SCBT QC
Date published: 2015-02-23
Rated 5 out of 5 by from Positive signal via western blot analysis Positive signal via western blot analysis of Cdc2 p34 expression in HeLa nuclear extract. -SCBT QC
Date published: 2013-04-26
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