Cdc13 Antibody (6F11/2): sc-53215
- Cdc13 Antibody (6F11/2) is a mouse monoclonal IgG2a Cdc13 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against S.pombe cdc13 whole protein
- Cdc13 Antibody (6F11/2) is recommended for detection of Cdc13 of S. pombe origin by WB and IP
- Anti-Cdc13 Antibody (6F11/2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Cdc13 Antibody (6F11/2). This work is in progress.
Cdc13 Antibody (6F11/2) is a mouse monoclonal IgG2a antibody that detects Cdc13 protein of Schizosaccharomyces pombe origin by western blotting (WB) and immunoprecipitation (IP). anti-Cdc13 antibody (6F11/2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cdc13 protein, consisting of 482 amino acids, plays a crucial role in regulating cell cycle, particularly during G2/M phase transition, where Cdc13 is essential for proper mitotic progression. Cdc13 protein is localized in the nucleus and serves as a key component of the cyclin family, which is vital for forming protein kinase complexes that control cell proliferation. Notably, Cdc13 undergoes post-translational modifications, including phosphorylation at specific serine residues (Ser 177, Ser 180, and Ser 183), which can influence activity and interactions with other proteins. Understanding these modifications helps reveal how phosphorylation affects Cdc13 stability and function, ultimately impacting cell cycle regulation and cytoskeletal reorganization.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Cdc13 Antibody (6F11/2) References:
- In vivo localisation of fission yeast cyclin-dependent kinase cdc2p and cyclin B cdc13p during mitosis and meiosis. | Decottignies, A., et al. 2001. J Cell Sci. 114: 2627-40. PMID: 11683390
- Activity of Cdc2 and its interaction with the cyclin Cdc13 depend on the molecular chaperone Cdc37 in Schizosaccharomyces pombe. | Turnbull, EL., et al. 2006. J Cell Sci. 119: 292-302. PMID: 16390871
- Involvement of cdc13+ in mitotic control in Schizosaccharomyces pombe: possible interaction of the gene product with microtubules. | Booher, R. and Beach, D. 1988. EMBO J. 7: 2321-7. PMID: 2847913
- Cloning and sequencing of the cyclin-related cdc13+ gene and a cytological study of its role in fission yeast mitosis. | Hagan, I., et al. 1988. J Cell Sci. 91 (Pt 4): 587-95. PMID: 2908246
- Two fission yeast B-type cyclins, cig2 and Cdc13, have different functions in mitosis. | Bueno, A. and Russell, P. 1993. Mol Cell Biol. 13: 2286-97. PMID: 8455610
- A quantitative model for the cdc2 control of S phase and mitosis in fission yeast. | Stern, B. and Nurse, P. 1996. Trends Genet. 12: 345-50. PMID: 8855663
- The role of proteolysis in cell cycle progression in Schizosaccharomyces pombe. | Yamano, H., et al. 1996. EMBO J. 15: 5268-79. PMID: 8895572
- The cdr2(+) gene encodes a regulator of G2/M progression and cytokinesis in Schizosaccharomyces pombe. | Breeding, CS., et al. 1998. Mol Biol Cell. 9: 3399-415. PMID: 9843577
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdc13 Antibody (6F11/2) | sc-53215 | 200 µg/ml | $322.00 | |||
Cdc13 Antibody (6F11/2) AC | sc-53215 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Cdc13 Antibody (6F11/2) HRP | sc-53215 HRP | 200 µg/ml | $322.00 | |||
Cdc13 Antibody (6F11/2) FITC | sc-53215 FITC | 200 µg/ml | $336.00 | |||
Cdc13 Antibody (6F11/2) PE | sc-53215 PE | 200 µg/ml | $349.00 | |||
Cdc13 Antibody (6F11/2) Alexa Fluor® 488 | sc-53215 AF488 | 200 µg/ml | $364.00 | |||
Cdc13 Antibody (6F11/2) Alexa Fluor® 546 | sc-53215 AF546 | 200 µg/ml | $364.00 | |||
Cdc13 Antibody (6F11/2) Alexa Fluor® 594 | sc-53215 AF594 | 200 µg/ml | $364.00 | |||
Cdc13 Antibody (6F11/2) Alexa Fluor® 647 | sc-53215 AF647 | 200 µg/ml | $364.00 | |||
Cdc13 Antibody (6F11/2) Alexa Fluor® 680 | sc-53215 AF680 | 200 µg/ml | $364.00 | |||
Cdc13 Antibody (6F11/2) Alexa Fluor® 790 | sc-53215 AF790 | 200 µg/ml | $364.00 |