Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD84 CRISPR/Cas9 KO Plasmid (h): sc-416482
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD84 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CD84 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD84 Antibody (152-1D5): sc-23899
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD84 CRISPR/Cas9 KO Plasmid (h) | sc-416482 | 20 µg | $397.00 |
Overview
CD84 is a SLAM family immunoglobulin superfamily receptor expressed predominantly on immune cells, where it participates in homophilic adhesion and co-stimulatory signaling that shapes lymphocyte activation and intercellular communication. Through associations with adaptor proteins such as SH2D1A/SAP and downstream kinases, CD84 influences signaling events linked to immune synapse formation, cytokine responses, and regulation of B cell and T cell interactions. Altered CD84 expression or signaling has been reported in immune dysregulation contexts, including inflammatory states and hematologic malignancy-associated immune phenotypes. As a surface immunoregulatory node, CD84 is frequently studied in pathways governing leukocyte activation, differentiation, and cell–cell contact-dependent signaling.
CD84 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CD84 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CD84 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CD84 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CD84 protein expression.
This CRISPR knockout system enables efficient generation of CD84-deficient cell models for investigation of CD84 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CD84 exon(s) critical for CD84 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CD84 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CD84 CRISPR/Cas9 KO Plasmid (h) and CD84 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CD84 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CD84 HDR Plasmid (h) and CD84 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CD84 homology arms to support homology-directed repair at defined CD84 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.