CD83 Antibody (D-3): sc-55536

CD83 Antibody (D-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects CD83 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). anti-CD83 antibody (D-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD83 is a heavily glycosylated membrane protein belonging to the immunoglobulin superfamily, primarily expressed in mature dendritic cells, Langerhans cells, and interdigitating reticulum cells within lymphoid tissues. CD83 plays a crucial role in immune responses, particularly in the activation and maturation of dendritic cells, which are essential for initiating T cell responses against pathogens and tumors. Structurally, CD83 features an extracellular V-type immunoglobulin-like domain, a single transmembrane domain, and a 40-residue cytoplasmic tail, which are important for its function in cell signaling and adhesion. CD83 has been identified as a sialic acid-binding immunoglobulin-like lectin (Siglec) adhesion molecule, facilitating interactions between dendritic cells and monocytes as well as CD8+ T cells, thereby enhancing the immune response. CD83 expression serves as a marker for mature antigen-presenting dendritic cells, which are capable of generating tumor-specific T cell immunity, highlighting its potential as a target for anti-cancer vaccines.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CD83 Antibody (D-3) References:

1. Diverse functional activity of CD83+ monocyte-derived dendritic cells and the implications for cancer vaccines.  |  Czerniecki, BJ., et al. 2001. Crit Rev Immunol. 21: 157-78. PMID: 11642602
2. CD83-stimulated monocytes suppress T-cell immune responses through production of prostaglandin E2.  |  Chen, L., et al. 2011. Proc Natl Acad Sci U S A. 108: 18778-83. PMID: 22065790
3. Targeting CD83 for the treatment of graft-versus-host disease.  |  Wang, X., et al. 2013. Exp Ther Med. 5: 1545-1550. PMID: 23837028
4. CD83 is a new potential biomarker and therapeutic target for Hodgkin lymphoma.  |  Li, Z., et al. 2018. Haematologica. 103: 655-665. PMID: 29351987
5. CD83 expression is essential for Treg cell differentiation and stability.  |  Doebbeler, M., et al. 2018. JCI Insight. 3: PMID: 29875316
6. Soluble CD83 alleviates experimental allergic rhinitis through modulating antigen-specific Th2 cell property.  |  Wu, YJ., et al. 2020. Int J Biol Sci. 16: 216-227. PMID: 31929750
7. Epithelial cell-derived CD83 restores immune tolerance in the airway mucosa by inducing regulatory T-cell differentiation.  |  Mo, LH., et al. 2021. Immunology. 163: 310-322. PMID: 33539546
8. Intestinal Epithelial Cell-Derived CD83 Contributes to Regulatory T-Cell Generation and Inhibition of Food Allergy.  |  Yu, Y., et al. 2021. J Innate Immun. 13: 295-305. PMID: 34182560
9. Tolerogenic IDO1+CD83- Langerhans Cells in Sentinel Lymph Nodes of Patients with Melanoma.  |  Gerlini, G., et al. 2022. Int J Mol Sci. 23: PMID: 35408802
10. RNA- and ATAC-sequencing Reveals a Unique CD83+ Microglial Population Focally Depleted in Parkinson's Disease.  |  Chatila, ZK., et al. 2023. bioRxiv.. PMID: 37292857

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD83 Antibody (D-3) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-CD83 Antibody (D-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD83 Antibody (D-3) Specifications

Alternate Product Name: CD83

Product Clone Name: D-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 7-205

Epitope Target: CD83

Epitope Species: human

Product Reactivity/Detection: CD83

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for CD83 Antibody (D-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred CD83 antibody is CD83 Antibody (HB15a): sc-19677.