CD8-alpha Antibody (C8/144B): sc-53212

CD8-α Antibody (C8/144B) is a mouse monoclonal IgG1 kappa light chain antibody that specifically detects the CD8-α protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). CD8-α, also known as CD8A, T8, or Leu-2, is a transmembrane glycoprotein expressed on the surface of cytotoxic T lymphocytes and some natural killer cells, playing a crucial role in the adaptive immune response. Post-translational modifications of CD8-α, such as glycosylation and phosphorylation, are essential for proper folding, stability, and function, influencing interaction with the T cell receptor (TCR) and major histocompatibility complex (MHC) class I molecules. These modifications can affect signal transduction pathways and the efficacy of T cell activation, making them important for understanding immune responses and developing therapeutic interventions. The study of CD8-α is pivotal for understanding adaptive immune responses, autoimmune conditions, and the development of novel therapeutic strategies, making anti-CD8-α antibody (C8/144B) an invaluable tool for researchers aiming to investigate T cell-mediated immunity and its implications in various disease models. CD8-α monoclonal antibody (C8/144B) is available in non-conjugated form as well as various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, to suit a wide range of experimental applications.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CD8-alpha Antibody (C8/144B) References:

1. Memory phenotype of CD8+ T cells in MHC class Ia-deficient mice.  |  Kurepa, Z., et al. 2003. J Immunol. 170: 5414-20. PMID: 12759416
2. CD8 alpha- and Langerin-negative dendritic cells, but not Langerhans cells, act as principal antigen-presenting cells in leishmaniasis.  |  Ritter, U., et al. 2004. Eur J Immunol. 34: 1542-50. PMID: 15162423
3. Differential expression of CD8 alpha and CD8 beta associated with MHC-restricted and non-MHC-restricted cytolytic effector cells.  |  Baume, DM., et al. 1990. Cell Immunol. 131: 352-65. PMID: 2122925
4. Phenotypic heterogeneity of persistent expansions of CD4+ CD8+ T cells.  |  Tonutti, E., et al. 1994. Clin Immunol Immunopathol. 73: 312-20. PMID: 7955559
5. Requirement for CD8 beta chain in positive selection of CD8-lineage T cells.  |  Nakayama, K., et al. 1994. Science. 263: 1131-3. PMID: 8108731
6. Decreased CD8-p56lck activity in peripheral blood T-lymphocytes from patients with hereditary haemochromatosis.  |  Arosa, FA., et al. 1994. Scand J Immunol. 39: 426-32. PMID: 8191217
7. Evidence for differential intracellular signaling via CD4 and CD8 molecules.  |  Ravichandran, KS. and Burakoff, SJ. 1994. J Exp Med. 179: 727-32. PMID: 8294879
8. Functional GATA-3 binding sites within murine CD8 alpha upstream regulatory sequences.  |  Landry, DB., et al. 1993. J Exp Med. 178: 941-9. PMID: 8350061
9. The TCR-beta chain repertoire of gut-derived T lymphocytes.  |  Regnault, A., et al. 1995. Semin Immunol. 7: 307-19. PMID: 8580463
10. Differential expression of CD8 epitopes amongst porcine CD8-positive functional lymphocyte subsets.  |  Yang, H. and Parkhouse, RM. 1997. Immunology. 92: 45-52. PMID: 9370923

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD8-α Antibody (C8/144B) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-CD8-α Antibody (C8/144B) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD8-α Antibody (C8/144B) Specifications

Alternate Product Name: CD8-α

Product Clone Name: C8/144B

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: CD8-α

Epitope Species: human

Product Reactivity/Detection: CD8-α

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for CD8-α Antibody (C8/144B): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred CD8-α antibody is CD8-α Antibody (53-6.7): sc-18913.