CD79B Antibody (B29/123) recommended for detection of CD79B of mouse, rat, human, porcine and equine origin by WB, IP, IF and IHC(P)

CD79B Antibody (B29/123): sc-53210

CD79B Antibody (B29/123) is rated 5.0 out of 5 by 6.
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Datasheets

    • Anti-CD79B Antibody (B29/123) is a mouse monoclonal IgG2b (lambda light chain) CD79B antibody provided at 200 µg/ml
    • raised against C-terminal 215 amino acids of murine B29
    • Anti-CD79B Antibody (B29/123) is recommended for detection of CD79B of mouse, rat, human, porcine and equine origin by WB, IP, IF and IHC(P)
    • Anti-CD79B Antibody (B29/123) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD79B (B29/123): sc-53210.

I am using CD79B (B29/123): sc-53210 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jenni
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Strong band in WB in human transfected 293T Strong band in WB in human transfected 293T and Raji whole cell lysates. -SCBT QC
Date published: 2015-06-08
Rated 5 out of 5 by from Worked well in WB in Ramos whole cell lysate Worked well in WB in Ramos whole cell lysate. -SCBT QC
Date published: 2015-05-24
Rated 5 out of 5 by from Produced excellent immunoperoxidase cytoplasmic Produced excellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human spleen tissue. -SCBT QC
Date published: 2015-04-18
Rated 5 out of 5 by from Robust signal in WB in NAMALWA Robust signal in WB in NAMALWA, Ramos, Raji and Daudi whole cell lysates. -SCBT QC
Date published: 2015-04-16
Rated 5 out of 5 by from Positive signal in WB in Daudi whole cell lysate Positive signal in WB in Daudi whole cell lysate. -SCBT QC
Date published: 2015-03-04
Rated 5 out of 5 by from Strong staining in IF in mouse WEH1231 cells Strong staining in IF in mouse WEH1231 cells. -SCBT Publication Review
Date published: 2015-02-19
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