CD71/TFRC/Transferrin Receptor Antibody (3H3074): sc-70778

CD71 Antibody (3H3074) is a rat monoclonal IgG2a antibody that detects CD71 in mouse and rat samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). CD71, also known as the transferrin receptor (TFR), plays a crucial role in iron homeostasis by facilitating cellular uptake of iron through receptor-mediated endocytosis, which is vital for various biological processes, including erythropoiesis and cellular proliferation. This type II membrane glycoprotein exists as a disulfide-linked homodimer, allowing binding to two molecules of transferrin, a serum iron-transport protein. CD71 is predominantly expressed on proliferating cells, reticulocytes, and erythroid precursors, while expression is notably absent in resting leukocytes but can be upregulated upon activation of lymphocytes, monocytes, and macrophages. CD71 is present on most dividing cells and brain endothelium, highlighting importance in both immune response and central nervous system function. The presence of a second transferrin receptor, TFR2, which also mediates transferrin-bound iron uptake, further emphasizes the complexity of iron regulation in the body. Mutations in the TFR2 gene can lead to hereditary hemochromatosis type III (HFE3), an iron overload disorder that predominantly affects Caucasians, underscoring the clinical significance of understanding CD71′s function and regulation.

The preferred CD71/TFRC/Transferrin Receptor antibody is CD71/TFRC/Transferrin Receptor Antibody (3B8 2A1): sc-32272.