CD69 Antibody (HP-4B3): sc-65270

CD69 Antibody (HP-4B3) is a mouse monoclonal IgG2a antibody that detects CD69 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). CD69 is a crucial activation marker expressed as a disulfide-linked homodimer known as the activation inducer molecule (AIM), which consists of two differentially glycosylated forms of a single protein. Anti-CD69 antibody (HP-4B3) recognizes a protein that plays a significant role in the immune response, as CD69 is one of the earliest antigens to appear following the activation of T cells, B cells, and natural killer (NK) cells. CD69 expression on activated immune cells is essential for regulating proliferation and differentiation, thereby influencing the overall immune response. Notably, CD69 is constitutively expressed on platelets, CD4+ or CD8+ thymocytes, and germinal center T cells, while remaining absent from resting lymphocytes, highlighting CD69′s importance as a marker for cellular activation. CD69 monoclonal antibody (HP-4B3) effectively identifies this activation marker in various applications, making this reagent valuable for researchers studying immune cell activation and function.

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CD69 Antibody (HP-4B3) References:

1. CD69 is an immunoregulatory molecule induced following activation.  |  Sancho, D., et al. 2005. Trends Immunol. 26: 136-40. PMID: 15745855
2. CD69 controls the uptake of L-tryptophan through LAT1-CD98 and AhR-dependent secretion of IL-22 in psoriasis.  |  Cibrian, D., et al. 2016. Nat Immunol. 17: 985-96. PMID: 27376471
3. CD69 Plays a Beneficial Role in Ischemic Stroke by Dampening Endothelial Activation.  |  Brait, VH., et al. 2019. Circ Res. 124: 279-291. PMID: 30582456
4. The Clinical Significance of Hepatic CD69+ CD103+ CD8+ Resident-Memory T Cells in Autoimmune Hepatitis.  |  You, Z., et al. 2021. Hepatology. 74: 847-863. PMID: 33554350
5. CD69 flow cytometry to complement interferon-γ release assay for active tuberculosis.  |  Kim, Y., et al. 2022. Cytometry B Clin Cytom. 102: 471-486. PMID: 36161692
6. Pre-transplant CD69+ extracellular vesicles are negatively correlated with active ATLG serum levels and associate with the onset of GVHD in allogeneic HSCT patients.  |  Storci, G., et al. 2022. Front Immunol. 13: 1058739. PMID: 36713433
7. Lung-resident CD69+ST2+ TH2 cells mediate long-term type 2 memory to inhaled antigen in mice.  |  Kobayashi, T., et al. 2023. J Allergy Clin Immunol. 152: 167-181.e6. PMID: 36720287
8. CD69 serves as a potential diagnostic and prognostic biomarker for hepatocellular carcinoma.  |  Tang, K., et al. 2023. Sci Rep. 13: 7452. PMID: 37156819
9. IRG1/Itaconate inhibits proliferation and promotes apoptosis of CD69+CD103+CD8+ tissue-resident memory T cells in autoimmune hepatitis by regulating the JAK3/STAT3/P53 signalling pathway.  |  Zhou, P., et al. 2024. Apoptosis. 29: 1738-1756. PMID: 38641760
10. CD69+ Vδ1γδ T cells are anti-tumor subpopulations in hepatocellular carcinoma.  |  You, H., et al. 2024. Mol Immunol. 172: 76-84. PMID: 38917598

The preferred CD69 antibody is CD69 Antibody (D-3): sc-373799.