CD68 Antibody (E-11) recommended for detection of CD68 of human origin by WB, IP, IF, IHC(P), FCM and ELISA

CD68 Antibody (E-11): sc-17832

CD68 Antibody (E-11) is rated 4.9 out of 5 by 7.
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Datasheets

    • CD68 Antibody (E-11) is a mouse monoclonal IgG1 κ, cited in 13 publications, provided at 200 µg/ml
    • raised against amino acids 100-354 of CD68 of human origin
    • CD68 Antibody (E-11) is recommended for detection of CD68 of human origin by WB, IP, IF, IHC(P), FCM and ELISA
    • Anti-CD68 Antibody (E-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD68 (E-11): sc-17832.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgG1 BP-HRP (mouse IgG1 binding protein-HRP) are the preferred secondary detection reagents for CD68 Antibody (E-11) for WB and IHC(P) applications. These reagents are now offered in bundles with CD68 Antibody (E-11) (see ordering information below).

Can CD68 (E-11): sc-17832 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Cweed
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD68 (E-11): sc-17832 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from good We use this antibody for Western blot, the blot are wonderful and got bands very clear.
Date published: 2017-05-10
Rated 5 out of 5 by from CD68 Antibody CD68 Antibody (E-11) produced great clean signal in WB.
Date published: 2017-05-07
Rated 5 out of 5 by from Versatile antibody Excellent results with CD68 Antibody (E-11)! Specific, clean and strong results by WB, IHCP, IF and FCM
Date published: 2017-01-25
Rated 5 out of 5 by from Produced excellent immunofluorescence cytoplasmic Produced excellent immunofluorescence cytoplasmic and membrane staining in methanol-fixed thp-1 cells. -SCBT QC
Date published: 2015-05-18
Rated 4 out of 5 by from Robust size band obtained in THP Robust size band obtained in THP-1 whole cell lysate. -SCBT QC
Date published: 2015-04-25
Rated 5 out of 5 by from Single strong band at correct mw in U Single strong band at correct mw in U-937 whole cell lysate. -SCBT QC
Date published: 2015-02-25
Rated 5 out of 5 by from Exceptional FCM data of PMA Exceptional FCM data of PMA-stimulated human peripheral blood leukocytes. -SCBT QC
Date published: 2013-09-10
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