CD59 Antibody (YTH53.1): sc-59095
- CD59 Antibody (YTH53.1) is a rat monoclonal IgG2b, cited in 12 publications, provided at 200 µg/ml
- raised against peripheral blood T cells of human origin
- Anti-CD59 Antibody (YTH53.1) is recommended for detection of CD59 of mouse, rat and human origin by WB, IP, IF and FCM
- Anti-CD59 Antibody (YTH53.1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for CD59 Antibody (YTH53.1). This work is in progress.
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CD59 Antibody (YTH53.1) is a rat monoclonal IgG2b antibody that detects CD59 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). CD59 Antibody (YTH53.1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD59 is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein predominantly located on the surface of leukocytes, vascular endothelial cells, various epithelial cells, and the placenta, playing a crucial role in immune regulation. CD59′s strategic location on cell membranes is vital as CD59 acts as a protective agent against the complement system, preventing the formation of the membrane attack complex (MAC) that can lead to cell lysis. By binding to the C8 and C9 components of the MAC, CD59 effectively inhibits the lytic pore formation, thereby safeguarding host cells from complement-mediated damage. Additionally, CD59 is involved in modulating calcium release and activating lipid-raft associated signaling molecules, including tyrosine kinases, which are essential for various cellular processes. The CD59 gene contains two p53-responsive domains, suggesting a potential role in protecting cells from complement-induced damage during inflammatory responses, and indicating that p53 may influence the susceptibility of tumor cells to complement lysis during chemotherapy.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CD59 Antibody (YTH53.1) References:
- Relating GPI-Anchored Ly6 Proteins uPAR and CD59 to Viral Infection. | Yu, J., et al. 2019. Viruses. 11: PMID: 31739586
- Association of Blood Group Antigen CD59 with Disease. | Weinstock, C. 2022. Transfus Med Hemother. 49: 13-24. PMID: 35221864
- Cd59 and inflammation regulate Schwann cell development. | Wiltbank, AT., et al. 2022. Elife. 11: PMID: 35748863
- CD59-Regulated Ras Compartmentalization Orchestrates Antitumor T-cell Immunity. | Li, L., et al. 2022. Cancer Immunol Res. 10: 1475-1489. PMID: 36206575
- Silencing EGFR-upregulated expression of CD55 and CD59 activates the complement system and sensitizes lung cancer to checkpoint blockade. | Shao, F., et al. 2022. Nat Cancer. 3: 1192-1210. PMID: 36271172
- CD59 Expression in Skeletal Muscles and Its Role in Myasthenia Gravis. | Iwasa, K., et al. 2023. Neurol Neuroimmunol Neuroinflamm. 10: PMID: 36396448
- Structural basis for membrane attack complex inhibition by CD59. | Couves, EC., et al. 2023. Nat Commun. 14: 890. PMID: 36797260
- Inherited CD59 deficiency, where neurology and genetics intertwine. | Almutawea, LM., et al. 2023. Neurosciences (Riyadh). 28: 130-135. PMID: 37045466
- Unveiling CD59-Antibody Interactions to Design Paratope-Mimicking Peptides for Complement Modulation. | Sandomenico, A., et al. 2023. Int J Mol Sci. 24: PMID: 37239905
- Dynamics and Molecular Interactions of GPI-Anchored CD59. | Voisin, TB., et al. 2023. Toxins (Basel). 15: PMID: 37505699
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD59 Antibody (YTH53.1) | sc-59095 | 200 µg/ml | $322.00 | |||
CD59 Antibody (YTH53.1) AC | sc-59095 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CD59 Antibody (YTH53.1) HRP | sc-59095 HRP | 200 µg/ml | $322.00 | |||
CD59 Antibody (YTH53.1) FITC | sc-59095 FITC | 200 µg/ml | $336.00 | |||
CD59 Antibody (YTH53.1) PE | sc-59095 PE | 200 µg/ml | $349.00 | |||
CD59 Antibody (YTH53.1) Alexa Fluor® 488 | sc-59095 AF488 | 200 µg/ml | $364.00 | |||
CD59 Antibody (YTH53.1) Alexa Fluor® 546 | sc-59095 AF546 | 200 µg/ml | $364.00 | |||
CD59 Antibody (YTH53.1) Alexa Fluor® 594 | sc-59095 AF594 | 200 µg/ml | $364.00 | |||
CD59 Antibody (YTH53.1) Alexa Fluor® 647 | sc-59095 AF647 | 200 µg/ml | $364.00 | |||
CD59 Antibody (YTH53.1) Alexa Fluor® 680 | sc-59095 AF680 | 200 µg/ml | $364.00 | |||
CD59 Antibody (YTH53.1) Alexa Fluor® 790 | sc-59095 AF790 | 200 µg/ml | $364.00 |