CD59 Antibody (1B4): sc-53626
- CD59 Antibody (1B4) is a mouse monoclonal mouse IgG2b κ κ provided at 200 µg/ml
- raised against CD59 of rat origin
- recommended for detection of CD59 of mouse and rat origin by WB, IP and FCM; not recommended for detection of human CD59
- available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for CD59 Antibody (1B4). This work is in progress.
QUICK LINKS
SEE ALSO...
CD59 Antibody (1B4) is a mouse monoclonal IgG2b antibody that detects CD59 in mouse and rat samples through applications such as western blotting (WB), immunoprecipitation (IP), and flow cytometry (FCM). CD59 is a glycosylphosphatidylinositol (GPI)-anchored protein predominantly located on the surface of various cell types, including leukocytes, vascular endothelial cells, and epithelial cells, as well as in the placenta. Strategic positioning on the cell membrane is crucial for CD59′s role in regulating the complement system, as CD59 prevents the formation of the membrane attack complex (MAC) that can lead to cell lysis. By binding to the C8 and C9 components of the MAC, CD59 effectively protects host cells from complement-mediated damage, which is particularly important during inflammatory responses. Additionally, CD59 is involved in signaling pathways that promote calcium release and activate lipid-raft associated signaling molecules, such as tyrosine kinases, further underscoring CD59′s significance in cellular communication and immune responses. The CD59 gene contains two p53-responsive domains, suggesting a potential link between CD59 expression and cellular defense mechanisms against complement-mediated damage, which may have implications for tumor cell susceptibility during chemotherapy. Anti-CD59 antibody (1B4) is an essential tool for researchers studying immune regulation and complement system interactions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CD59 Antibody (1B4) References:
- Defining the CD59-C9 binding interaction. | Huang, Y., et al. 2006. J Biol Chem. 281: 27398-404. PMID: 16844690
- CD59 mediates cartilage patterning during spontaneous tail regeneration. | Bai, X., et al. 2015. Sci Rep. 5: 12798. PMID: 26238652
- CD59 Regulation by SOX2 Is Required for Epithelial Cancer Stem Cells to Evade Complement Surveillance. | Chen, J., et al. 2017. Stem Cell Reports. 8: 140-151. PMID: 28017655
- CD59 association with infectious bronchitis virus particles protects against antibody-dependent complement-mediated lysis. | Wei, Y., et al. 2017. J Gen Virol. 98: 2725-2730. PMID: 29068273
- Molecular pathogenesis of human CD59 deficiency. | Karbian, N., et al. 2018. Neurol Genet. 4: e280. PMID: 30533526
- Endovascular trophoblast expresses CD59 to evade complement-dependent cytotoxicity. | Ueda, M., et al. 2019. Mol Cell Endocrinol. 490: 57-67. PMID: 30981734
- A Splice Site Mutation Associated with Congenital CD59 Deficiency. | Chai, JN., et al. 2022. Hematol Rep. 14: 172-178. PMID: 35735736
- The bioinformatics analysis of CD59 in Helicobacter pylori infected gastric cancer. | Sun, J., et al. 2023. Cancer Biomark. 38: 27-35. PMID: 37522198
- Glycated CD59 is a potential biomarker for gestational diabetes mellitus. | Wang, W., et al. 2024. Front Endocrinol (Lausanne). 15: 1374253. PMID: 39351537
- Structure, distribution, and functional role of protectin (CD59) in complement-susceptibility and in immunotherapy of human malignancies (Review). | Maio, M., et al. 1998. Int J Oncol. 13: 305-18. PMID: 9664126
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD59 Antibody (1B4) | sc-53626 | 200 µg/ml | $322.00 | |||
CD59 Antibody (1B4) FITC | sc-53626 FITC | 200 µg/ml | $336.00 | |||
CD59 Antibody (1B4) PE | sc-53626 PE | 200 µg/ml | $349.00 |