CD52 CRISPR/Cas9 KO Plasmid (h): sc-403151
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD52 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CD52 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD52 Antibody (HI186): sc-51560
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD52 CRISPR/Cas9 KO Plasmid (h) | sc-403151 | 20 µg | $397.00 |
Overview
CD52 is a glycosylphosphatidylinositol (GPI)-anchored surface glycoprotein highly expressed on mature lymphocytes, where it contributes to membrane organization and immune cell signaling within lipid rafts. Although it has a short peptide core, CD52 carries dense glycosylation that can influence receptor clustering, cell–cell interactions, and responsiveness to activation cues in hematopoietic lineages. CD52 expression is closely linked to lymphocyte differentiation and immune homeostasis, making it a useful marker in studies of immune cell states and leukocyte biology. Dysregulated CD52 expression and immune context associations have been reported across hematologic malignancies and inflammatory settings, supporting its relevance for mechanistic investigation of immune regulation.
CD52 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CD52 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CD52 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CD52 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CD52 protein expression.
This CRISPR knockout system enables efficient generation of CD52-deficient cell models for investigation of CD52 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CD52 exon(s) critical for CD52 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CD52 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CD52 CRISPR/Cas9 KO Plasmid (h) and CD52 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CD52 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CD52 HDR Plasmid (h) and CD52 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CD52 homology arms to support homology-directed repair at defined CD52 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.