CD52 Antibody (HI186): sc-51560

CD52 Antibody (HI186) is a mouse monoclonal IgG2b kappa light chain antibody that detects CD52 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). CD52 (HI186) antibody is available in both non-conjugated and various conjugated forms, including phycoerythrin (PE) and fluorescein isothiocyanate (FITC). CD52 protein is a glycosylphosphatidylinositol (GPI)-anchored surface antigen predominantly expressed on epithelial cells of the male reproductive tract, as well as on thymocytes, lymphocytes, monocytes, and macrophages, with variable expression in lymphoid malignancies. CD52 presence on the surface of epididymal sperm and in seminal plasma is crucial for sperm maturation, as spermatozoa acquire CD52 during passage through the genital tract, which is shed from the epithelial lining. CD52 is absent on spermatogenic cells and testicular spermatozoa, highlighting a specific role in later stages of sperm development. CD52 structure, characterized by a peptide backbone of 12 amino acids, serves primarily as a scaffold for essential post-translational modifications, including GPI anchoring and N-glycosylation, which are vital for stability and function in cellular interactions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CD52 Antibody (HI186) References:

1. CD52 antigen--a review.  |  Domagała, A. and Kurpisz, M. 2001. Med Sci Monit. 7: 325-31. PMID: 11257744
2. Expression of CD52 on plasma cells in plasma cell proliferative disorders.  |  Kumar, S., et al. 2003. Blood. 102: 1075-7. PMID: 12714489
3. First total synthesis of a GPI-anchored peptide.  |  Xue, J., et al. 2003. J Org Chem. 68: 4020-9. PMID: 12737586
4. Chemical synthesis of CD52 glycopeptides containing the acid-labile fucosyl linkage.  |  Shao, N., et al. 2003. J Org Chem. 68: 9003-11. PMID: 14604374
5. Possible presence of O-linked carbohydrate in the human male reproductive tract CD52.  |  Hasegawa, A., et al. 2004. J Reprod Immunol. 62: 91-100. PMID: 15288185
6. Antigenic epitope for sperm-immobilizing antibody detected in infertile women.  |  Hasegawa, A. and Koyama, K. 2005. J Reprod Immunol. 67: 77-86. PMID: 16107278
7. Different glycoforms of the human GPI-anchored antigen CD52 associate differently with lipid microdomains in leukocytes and sperm membranes.  |  Ermini, L., et al. 2005. Biochem Biophys Res Commun. 338: 1275-83. PMID: 16266689
8. CD52-targeted depletion by Alemtuzumab ameliorates allergic airway hyperreactivity and lung inflammation.  |  Shafiei-Jahani, P., et al. 2021. Mucosal Immunol. 14: 899-911. PMID: 33731828
9. CD52 mRNA expression predicts prognosis and response to immune checkpoint blockade in melanoma.  |  de Vos-Hillebrand, L., et al. 2024. Pigment Cell Melanoma Res. 37: 309-315. PMID: 37975535
10. Surface CD52, CD84, and PTGER2 mark mature PMN-MDSCs from cancer patients and G-CSF-treated donors.  |  Pettinella, F., et al. 2024. Cell Rep Med. 5: 101380. PMID: 38242120
11. T-cell dysfunction in CLL is mediated through expression of Siglec-10 ligands CD24 and CD52 on CLL cells.  |  van Bruggen, JAC., et al. 2024. Blood Adv. 8: 4633-4646. PMID: 39042920
12. Human epididymal secreted protein CD52 on ejaculated spermatozoa: correlations with semen characteristics and the effect of its antibody.  |  Yeung, CH., et al. 1997. Mol Hum Reprod. 3: 1045-51. PMID: 9464849

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD52 Antibody (HI186) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-CD52 Antibody (HI186) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD52 Antibody (HI186) Specifications

Alternate Product Name: CD52

Product Clone Name: HI186

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids:

Epitope Target: CD52

Epitope Species: human

Product Reactivity/Detection: precursor and mature CD52

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 100 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for CD52 Antibody (HI186): FITC: Fluoroscein, PE: Phycoerythrin