CD5 Antibody (UCH-T2): sc-1180
- CD5 Antibody (UCH-T2) is a mouse monoclonal IgG1 κ CD5 antibody, cited in 4 publications, provided at 200 µg/ml
- raised against human thymocytes/Sezary T cells
- CD5 Antibody (UCH-T2) is recommended for detection of CD5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
- Anti-CD5 Antibody (UCH-T2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CD5 Antibody (UCH-T2) for WB and IHC(P) applications. These reagents are now offered in bundles with CD5 Antibody (UCH-T2) (see ordering information below).
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CD5 Antibody (UCH-T2) is a mouse monoclonal IgG1 kappa light chain antibody that detects CD5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and flow cytometry (FCM). CD5 (UCH-T2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD5, also known as Lyt-1, is a crucial transmembrane glycoprotein predominantly expressed on approximately 70% of normal peripheral blood lymphocytes and nearly all T lymphocytes in the thymus and peripheral blood. CD5′s primary function involves modulating T cell activation; when T cells are activated through the T cell receptor (TCR), CD5 undergoes tyrosine phosphorylation, which is essential for regulating T cell responses. Notably, absence of CD5 leads to hyper-responsiveness of T cells to TCR-mediated activation, highlighting CD5′s role in maintaining immune homeostasis. CD5 interacts with the TCR/CD3-ζ chain and the Src family kinase Lck p56, significantly enhancing Lck′s kinase activity by 10- to 15-fold when bound to CD5. Additionally, CD5 serves as a receptor for the B cell antigen CD72, although the precise consequences of this interaction remain to be fully elucidated, suggesting a potential role in thymic selection processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CD5 Antibody (UCH-T2) References:
- CD5 is phosphorylated on tyrosine after stimulation of the T-cell antigen receptor complex. | Davies, AA., et al. 1992. Proc Natl Acad Sci U S A. 89: 6368-72. PMID: 1378627
- CD5+ B cells and the immune system. | Lydyard, PM., et al. 1993. Immunol Lett. 38: 159-66. PMID: 7507466
- The T-cell antigen CD5 acts as a receptor and substrate for the protein-tyrosine kinase p56lck. | Raab, M., et al. 1994. Mol Cell Biol. 14: 2862-70. PMID: 7513045
- Anti-CD5 therapy decreases severity of established disease in collagen type II-induced arthritis in DBA/1 mice. | Plater-Zyberk, C., et al. 1994. Clin Exp Immunol. 98: 442-7. PMID: 7527741
- A role for CD5 in TCR-mediated signal transduction and thymocyte selection. | Tarakhovsky, A., et al. 1995. Science. 269: 535-7. PMID: 7542801
- CD5 expression on B cells may be an activation marker for secretion of anti-myelin antibodies in patients with polyneuropathy associated with monoclonal gammopathy. | Ekerfelt, C., et al. 1995. Clin Exp Immunol. 101: 346-50. PMID: 7544252
- Expression of CD5 and CD72 on T and B cell subsets in rheumatoid arthritis and Sjögren's syndrome. | Jamin, C., et al. 1993. Clin Exp Immunol. 92: 245-50. PMID: 7683586
- Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies. | Jones, M., et al. 1993. J Immunol. 150: 5429-35. PMID: 8515069
- The structure of avian CD5 implies a conserved function. | Koskinen, R., et al. 1998. J Immunol. 160: 4943-50. PMID: 9590242
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD5 Antibody (UCH-T2) | sc-1180 | 200 µg/ml | $322.00 | |||
CD5 Antibody (UCH-T2): m-IgG Fc BP-HRP Bundle | sc-528123 | 200 µg Ab; 10 µg BP | $361.00 | |||
CD5 Antibody (UCH-T2): m-IgGκ BP-HRP Bundle | sc-520426 | 200 µg Ab, 40 µg BP | $361.00 | |||
CD5 Antibody (UCH-T2): m-IgG1 BP-HRP Bundle | sc-542775 | 200 µg Ab; 20 µg BP | $361.00 | |||
CD5 Antibody (UCH-T2) AC | sc-1180 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CD5 Antibody (UCH-T2) HRP | sc-1180 HRP | 200 µg/ml | $322.00 | |||
CD5 Antibody (UCH-T2) FITC | sc-1180 FITC | 200 µg/ml | $336.00 | |||
CD5 Antibody (UCH-T2) PE | sc-1180 PE | 200 µg/ml | $349.00 | |||
CD5 Antibody (UCH-T2) Alexa Fluor® 488 | sc-1180 AF488 | 200 µg/ml | $364.00 | |||
CD5 Antibody (UCH-T2) Alexa Fluor® 546 | sc-1180 AF546 | 200 µg/ml | $364.00 | |||
CD5 Antibody (UCH-T2) Alexa Fluor® 594 | sc-1180 AF594 | 200 µg/ml | $364.00 | |||
CD5 Antibody (UCH-T2) Alexa Fluor® 647 | sc-1180 AF647 | 200 µg/ml | $364.00 | |||
CD5 Antibody (UCH-T2) Alexa Fluor® 680 | sc-1180 AF680 | 200 µg/ml | $364.00 | |||
CD5 Antibody (UCH-T2) Alexa Fluor® 790 | sc-1180 AF790 | 200 µg/ml | $364.00 |