Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD48 CRISPR/Cas9 KO Plasmid (m): sc-419559
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD48 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CD48 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD48 Antibody (5-4.8): sc-8400
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD48 CRISPR/Cas9 KO Plasmid (m) | sc-419559 | 20 µg | $397.00 |
Overview
Mouse Cd48 encodes CD48, a glycosylphosphatidylinositol-anchored immunoglobulin superfamily receptor broadly expressed on hematopoietic cells that participates in immune cell adhesion and costimulation. CD48 engages CD2 and the SLAM family receptor 2B4 (CD244), shaping immunological synapse formation and tuning activation thresholds in T cells, NK cells, and antigen-presenting cells. Through these interactions, CD48 influences cytokine production, cytotoxic effector function, and leukocyte trafficking within inflammatory microenvironments. Dysregulated CD48 signaling and expression have been associated with altered immune homeostasis in models of autoimmunity, chronic inflammation, infection, and hematologic malignancy biology.
CD48 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Cd48 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cd48 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cd48 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CD48 protein expression.
This CRISPR knockout system enables efficient generation of Cd48-deficient cell models for investigation of CD48 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Cd48 exon(s) critical for CD48 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Cd48 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CD48 CRISPR/Cas9 KO Plasmid (m) and CD48 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cd48 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CD48 HDR Plasmid (m) and CD48 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cd48 homology arms to support homology-directed repair at defined Cd48 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.