CD48 Antibody (0.N.140): sc-70719

CD48 Antibody (0.N.140) is a mouse monoclonal IgG2a antibody that detects CD48 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). CD48 is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein that plays a crucial role in immune responses by acting as a low-affinity receptor for CD2, facilitating cell-cell interactions essential for T cell activation and proliferation. CD48 expression on various immune cells, including T, B, and null cells, as well as thymocytes and eosinophils, underscores CD48′s importance in modulating immune responses and maintaining homeostasis. CD48 associates with the tyrosine kinase Lck, which is vital for T cell receptor signaling, further emphasizing CD48′s significance in immune regulation. Additionally, CD48 expression on hematopoietic and endothelial cells in rats and on thymocytes and macrophages in mice highlights CD48′s diverse roles across different species, making anti-CD48 antibody (0.N.140) an invaluable tool for researchers studying immune function and cell signaling pathways.

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CD48 Antibody (0.N.140) References:

1. Molecular cloning and biological characterization of NK cell activation-inducing ligand, a counterstructure for CD48.  |  Kubin, MZ., et al. 1999. Eur J Immunol. 29: 3466-77. PMID: 10556801
2. Direct measurements of heterotypic adhesion between the cell surface proteins CD2 and CD48.  |  Zhu, B., et al. 2002. Biochemistry. 41: 12163-70. PMID: 12356317
3. NK cells stimulate proliferation of T and NK cells through 2B4/CD48 interactions.  |  Assarsson, E., et al. 2004. J Immunol. 173: 174-80. PMID: 15210772
4. Functional role played by the glycosylphosphatidylinositol anchor glycan of CD48 in interleukin-18-induced interferon-gamma production.  |  Fukushima, K., et al. 2005. J Biol Chem. 280: 18056-62. PMID: 15760905
5. Crystal structure and binding properties of the CD2 and CD244 (2B4)-binding protein, CD48.  |  Evans, EJ., et al. 2006. J Biol Chem. 281: 29309-20. PMID: 16803907
6. ORMDL3 promotes eosinophil trafficking and activation via regulation of integrins and CD48.  |  Ha, SG., et al. 2013. Nat Commun. 4: 2479. PMID: 24056518
7. A soluble multimeric recombinant CD2 protein identifies CD48 as a low affinity ligand for human CD2: divergence of CD2 ligands during the evolution of humans and mice.  |  Arulanandam, AR., et al. 1993. J Exp Med. 177: 1439-50. PMID: 7683037
8. A sensitive assay for detecting low-affinity interactions at the cell surface reveals no additional ligands for the adhesion pair rat CD2 and CD48.  |  Brown, MH., et al. 1995. Eur J Immunol. 25: 3222-8. PMID: 8566004
9. Preclinical antitumor activity of an antibody against the leukocyte antigen CD48.  |  Sun, H., et al. 1998. Clin Cancer Res. 4: 895-900. PMID: 9563883
10. 2B4, the natural killer and T cell immunoglobulin superfamily surface protein, is a ligand for CD48.  |  Brown, MH., et al. 1998. J Exp Med. 188: 2083-90. PMID: 9841922

The preferred CD48 antibody is CD48 Antibody (5-4.8): sc-8400.