CD47 Antibody (MIAP301): sc-12731
- CD47 Antibody (MIAP301) is a rat monoclonal IgG2a, cited in 22 publications, provided at 200 µg/ml
- raised against intact CD47 purified from placenta of mouse origin
- Anti-CD47 Antibody (MIAP301) is recommended for detection of the immunoglobulin domain of CD47 of mouse and rat origin by WB, IP, IF and FCM
- Anti-CD47 Antibody (MIAP301) is available conjugated to agarose for IP; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for CD47 Antibody (MIAP301). This work is in progress.
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SEE ALSO...
CD47 Antibody (MIAP301) is a rat monoclonal IgG2a antibody that detects the immunoglobulin domain of CD47 of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). CD47 Antibody (MIAP301) is available in both conjugated and non-conjugated forms, providing versatility for various experimental needs. CD47 is an integral membrane protein that plays a crucial role in regulating cation fluxes across cell membranes, which is vital for maintaining cellular homeostasis and signaling. Specifically, CD47 is involved in the increase of intracellular calcium concentration that occurs upon cell adhesion to the extracellular matrix, a process that is essential for various cellular functions, including migration, proliferation, and survival. Additionally, CD47 serves as a receptor for the C-terminal cell binding domain of thrombospondin, which is important for mediating cell-cell interactions and influencing immune responses. Notably, CD47 is absent from Rh-null erythrocytes, yet CD47 plays a significant role in cell adhesion in non-erythroid cells and may help prevent the premature elimination of erythrocytes. Furthermore, CD47 is expressed on a variety of cell types, including hemopoietic cells, epithelial cells, endothelial cells, and fibroblasts, with particularly strong expression in brain and mesenchymal cells, highlighting CD47′s importance in both normal physiology and pathological conditions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD47 Antibody (MIAP301) | sc-12731 | 200 µg/ml | $316.00 | |||
CD47 Antibody (MIAP301) AC | sc-12731 AC | 500 µg/ml, 25% agarose | $416.00 | |||
CD47 Antibody (MIAP301) FITC | sc-12731 FITC | 200 µg/ml | $330.00 | |||
CD47 Antibody (MIAP301) PE | sc-12731 PE | 200 µg/ml | $343.00 | |||
CD47 Antibody (MIAP301) Alexa Fluor® 488 | sc-12731 AF488 | 200 µg/ml | $357.00 | |||
CD47 Antibody (MAIP301) Alexa Fluor® 546 | sc-12731 AF546 | 200 µg/ml | $357.00 | |||
CD47 Antibody (MIAP301) Alexa Fluor® 594 | sc-12731 AF594 | 200 µg/ml | $357.00 | |||
CD47 Antibody (MIAP301) Alexa Fluor® 647 | sc-12731 AF647 | 200 µg/ml | $357.00 | |||
CD47 Antibody (MIAP301) Alexa Fluor® 680 | sc-12731 AF680 | 200 µg/ml | $357.00 | |||
CD47 Antibody (MIAP301) Alexa Fluor® 790 | sc-12731 AF790 | 200 µg/ml | $357.00 |