CD45RO Antibody (2Q1392): sc-70712

CD45RO Antibody (2Q1392) is a mouse monoclonal IgG2a kappa light chain antibody raised against a cultured human T cell line (CA-1) and reacts with mouse, rat, and human species. CD45RO monoclonal antibody (2Q1392) is validated for applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). CD45RO, also known as protein tyrosine phosphatase receptor type C (PTPRC), is a transmembrane glycoprotein broadly expressed in hematopoietic cells. CD45RO (2Q1392) antibody recognizes one of several isoforms of CD45 generated through alternative splicing, with CD45RO lacking exons 4, 5, and 6, resulting in a distinct extracellular domain while maintaining a conserved intracellular domain. CD45RO is primarily localized to the plasma membrane, where CD45RO functions as a phosphotyrosine phosphatase crucial for T cell receptor (TCR) signaling. This membrane localization allows CD45RO to regulate the activation of signaling molecules such as Src family kinases (Lck and Fyn) and Syk family kinases (ZAP-70), which are key mediators of T cell activation and immune response. CD45RO (2Q1392) monoclonal antibody serves as an essential tool for studying immune cell signaling, the role of CD45 isoforms, and their contributions to immune regulation and disease.

The preferred CD45 antibody is CD45 Antibody (35-Z6): sc-1178.