CD43 Antibody (DF-T1): sc-6256

CD43 Antibody (DF-T1) is a mouse monoclonal IgG1 kappa light chain antibody that detects CD43 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). CD43 Antibody (DF-T1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD43, also known as leukosialin, is a major O-glycosylated sialoglycoprotein predominantly found on the surface of leukocytes, playing a crucial role in cellular adhesion and tumor progression. CD43 is a member of the surface mucin family and is characterized by extensive glycosylation, which is essential for function in modulating cell-cell interactions and immune responses. CD43 glycosylation not only influences structural properties but also affects interactions with other proteins, thereby impacting processes such as leukocyte migration and activation. CD43 is particularly important in the context of T cells and certain B cell populations, serving as a key marker for identifying both normal and neoplastic cells in various tissues. By functioning as a negative regulator of cellular adhesion, CD43 helps to maintain the balance of immune responses, making CD43 a significant target for research in immunology and cancer biology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CD43 Antibody (DF-T1) References:

1. [Phenotypical and functional characterization of leukocytes--the CD-system].  |  Holter, W., et al. 1991. Wien Klin Wochenschr. 103: 247-62. PMID: 2068816
2. CD43 in the nucleus and cytoplasm of lung cancer is a potential therapeutic target.  |  Fu, Q., et al. 2013. Int J Cancer. 132: 1761-70. PMID: 23015282
3. Cancer-associated CD43 glycoforms as target of immunotherapy.  |  Tuccillo, FM., et al. 2014. Mol Cancer Ther. 13: 752-62. PMID: 24356816
4. Aberrant glycosylation as biomarker for cancer: focus on CD43.  |  Tuccillo, FM., et al. 2014. Biomed Res Int. 2014: 742831. PMID: 24689054
5. CD43 in the malignant flow cytometry laboratory in 2020.  |  Sorigue, M. 2021. Expert Rev Hematol. 14: 123-136. PMID: 33249940
6. CD11/CD18 panel report for swine CD workshop.  |  Kim, YB., et al. 1994. Vet Immunol Immunopathol. 43: 289-91. PMID: 7531911
7. Regulatory role of CD43 leukosialin on integrin-mediated T-cell adhesion to endothelial and extracellular matrix ligands and its polar redistribution to a cellular uropod.  |  Sánchez-Mateos, P., et al. 1995. Blood. 86: 2228-39. PMID: 7545023
8. Negative regulation of T-cell adhesion and activation by CD43.  |  Manjunath, N., et al. 1995. Nature. 377: 535-8. PMID: 7566153
9. Expression of the leukocyte-associated sialoglycoprotein CD43 by a colon carcinoma cell line.  |  Baeckström, D., et al. 1995. J Biol Chem. 270: 13688-92. PMID: 7775421
10. CD43 and CD5 antibodies define four normal and neoplastic B-cell subsets: a three-color flow cytometric study.  |  Lynch, EF., et al. 1995. Cytometry. 22: 223-31. PMID: 8556954
11. Specific monoclonal antibodies against leukocyte-restricted cell surface molecule CD43 react with nonhematopoietic tumor cells.  |  Santamaría, M., et al. 1996. Cancer Res. 56: 3526-9. PMID: 8758921
12. The CD43 130-kD peripheral T-cell activation antigen is downregulated in thymic positive selection.  |  Ellies, LG., et al. 1996. Blood. 88: 1725-32. PMID: 8781428

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD43 Antibody (DF-T1) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-CD43 Antibody (DF-T1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD43 Antibody (DF-T1) Specifications

Alternate Product Name: CD43

Product Clone Name: DF-T1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: CD43

Epitope Species: human

Product Reactivity/Detection: CD43

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for CD43 Antibody (DF-T1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin