CD40 Antibody (HM40-3) recommended for detection of CD40 of mouse and rat origin by WB, IP, IF, IHC(P) and FCM

CD40 Antibody (HM40-3): sc-20010

CD40 Antibody (HM40-3) is rated 4.3 out of 5 by 3.
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Datasheets

    • CD40 Antibody (HM40-3) is a Armenian hamster monoclonal IgM provided at 200 µg/ml
    • raised against (BALB/c x NZB)F1 mouse-derived lymphoma WEHI-231 cells
    • recommended for detection of CD40 of mouse and rat origin by WB, IP, IF, IHC(P) and FCM
    • also provided azide-free for blocking of ligand binding and stimulation of B lymphocytes, sc-20010 L, 200 µg/0.1 ml
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD40 (HM40-3): sc-20010.

This antibody is giving another band at around 54kD (other than the 43kD band). The results are consistent. Is its another isoform of CD40, that it is detecting? Its a virus-induced CD40 expression that we are checking.

Asked by: DEBANJANA
Thank you for your question. According to the literature, CD40 is a glycoprotein that could be detected at higher molecular weights.
Answered by: Technical Support
Date published: 2019-05-25

To do the Western blot of this antibody, how can I get the HRP conjugated anti hamster IgM? Would do please tell me the order information if possible?

Asked by: Ying2626
CD40 Antibody (HM40-3) is a Armenian hamster monoclonal IgM. Unfortunately, we do not currently offer an isotype-specific secondary antibody that is anti-Armenian hamster IgM-HRP. You could try using our mouse anti-Armenian hamster IgG-HRP: sc-2789, which may also be suitable for detection of IgM.
Answered by: Technical Support
Date published: 2019-04-20

what if the antibody is kept at room temperature for more than 2 days accidentally? will it still work?

Asked by: debby
Thank you for the question. We suggest storing this antibody at +4°C - 8°C in the fridge. If stored accidentally at room temp. for 2 days the product likely will not be harmed. We still suggest evaluating the antibody with a positive control and verify the obtained data.
Answered by: Technical Support Europe
Date published: 2018-01-31

How can CD40 (HM40-3): sc-20010 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jerojero
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-20010 PE) or fluoroscein (sc-20010 FITC).
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Great WB data obtained using mouse peripheral Great WB data obtained using mouse peripheral blood lysate. -SCBT QC
Date published: 2015-06-28
Rated 4 out of 5 by from Good FCM data of WEHI Good FCM data of WEHI-231 cells. -SCBT QC
Date published: 2015-05-03
Rated 4 out of 5 by from Good immunoperoxidase membrane staining in Good immunoperoxidase membrane staining in formalin fixed, paraffin-embedded mouse lung tissue. -SCBT QC
Date published: 2015-03-17
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