CD40 Antibody (HM40-3): sc-20010

CD40 Antibody (HM40-3) is a hamster monoclonal IgM antibody that detects CD40 protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and flow cytometry (FCM). Anti-CD40 antibody (HM40-3) is available in a non-conjugated form, providing researchers with a versatile tool for studying the CD40 signaling pathway. CD40 plays a crucial role in the immune response, particularly in the activation and differentiation of B cells. CD40 is primarily located on the surface of B cells and interacts with CD40 ligand (CD40L) expressed on activated T cells, facilitating essential cell-to-cell communication. This interaction is vital for B cell activation, leading to clonal expansion and the production of antibodies. The importance of CD40 in immune regulation is underscored by its involvement in immunoglobulin class switching and the secretion of IgE, which are critical processes in the adaptive immune response. Furthermore, the CD40 signaling pathway is mediated by downstream effectors such as CRAF, which is part of a family of proteins that includes TRAF1 and TRAF2, known for their roles in regulating tumor necrosis factor receptor signaling and activating the NFκB transcription factors. Understanding the function and interactions of CD40 is essential for elucidating mechanisms of immune responses and developing therapeutic strategies for immune-related diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CD40 Antibody (HM40-3) References:

1. Molecular mechanisms of CD40 signaling.  |  Bishop, GA. and Hostager, BS. 2001. Arch Immunol Ther Exp (Warsz). 49: 129-37. PMID: 11348017
2. CD40 in human oral epithelia.  |  Villarroel Dorrego, M., et al. 2007. Oral Oncol. 43: 626-33. PMID: 17307023
3. CD40-CD40 ligand interactions in oxidative stress, inflammation and vascular disease.  |  Rizvi, M., et al. 2008. Trends Mol Med. 14: 530-8. PMID: 18977174
4. The multi-functionality of CD40L and its receptor CD40 in atherosclerosis.  |  Lievens, D., et al. 2009. Thromb Haemost. 102: 206-14. PMID: 19652870
5. The CD40-CD40L system in cardiovascular disease.  |  Pamukcu, B., et al. 2011. Ann Med. 43: 331-40. PMID: 21244217
6. Update on CD40 and CD154 blockade in transplant models.  |  Zhang, T., et al. 2015. Immunotherapy. 7: 899-911. PMID: 26268734
7. The CD40/CD40 ligand dyad and its downstream effector molecule ISG54 in relating acute neuroinflammation with persistent, progressive demyelination.  |  Hazra, B. and Das Sarma, J. 2024. IUBMB Life. 76: 313-331. PMID: 38116887
8. Poorly Differentiated Hepatocellular Carcinoma Cells Avoid Apoptosis by Interacting with T Cells via CD40-CD40 Ligand Linkage.  |  Vinh Hanh, N., et al. 2024. Am J Pathol. 194: 1230-1247. PMID: 38548267
9. CD40/CD40 ligand interactions in normal, reactive and malignant lympho-hematopoietic tissues.  |  Gruss, HJ., et al. 1997. Leuk Lymphoma. 24: 393-422. PMID: 9086433
10. CD40-CD40 ligand interaction in autoimmune disease.  |  Datta, SK. and Kalled, SL. 1997. Arthritis Rheum. 40: 1735-45. PMID: 9336404

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD40 Antibody (HM40-3) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-CD40 Antibody (HM40-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD40 Antibody (HM40-3) Specifications

Alternate Product Name: CD40

Product Clone Name: HM40-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM

Amino Acids:

Epitope Target: CD40

Epitope Species: mouse

Product Reactivity/Detection: CD40

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgM in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for CD40 Antibody (HM40-3): L: Azide-Free

The preferred CD40 antibody is CD40 Antibody (H-10): sc-13128.