Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Resting B cells can be activated and clonally expanded into antibody-producing cells in response to a combination of cell contact and soluble signals provided by primed helper T (Th) cells. While cytokines IL-4 and IL-13 alone are inadequate for B cell activation, contact with Th cells seems to be sufficient for delivery of proliferative signals. A receptor ligand pair central to the transmission of this signal is CD40, expressed on the surface of B cells, together with CD40L, expressed on activated T cells. In the presence of such stimulus, IL-4 and IL-13 are capable of triggering immunoglobulin class switching and secretion of IgE. B cells are sensitive to these cytokines only subsequent to CD40/CD40L-driven DNA synthesis. A downstream mediator of the CD40 signaling pathway, designated CRAF, is a member of an expanding family of proteins that contain a conserved cysteine- and histidine-rich RING finger motif. Other members of the family include TRAF1 and TRAF2. The latter proteins have been shown to regulate TNF-R2 as well as CD40 signaling through activation of the NFkB family of transcription factors.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
This antibody is giving another band at around 54kD (other than the 43kD band). The results are consistent. Is its another isoform of CD40, that it is detecting? Its a virus-induced CD40 expression that we are checking.
Asked by: DEBANJANA
Thank you for your question. According to the literature, CD40 is a glycoprotein that could be detected at higher molecular weights.
Answered by: Technical Support
Date published: 2019-05-25
To do the Western blot of this antibody, how can I get the HRP conjugated anti hamster IgM? Would do please tell me the order information if possible?
Asked by: Ying2626
CD40 Antibody (HM40-3) is a Armenian hamster monoclonal IgM. Unfortunately, we do not currently offer an isotype-specific secondary antibody that is anti-Armenian hamster IgM-HRP. You could try using our mouse anti-Armenian hamster IgG-HRP: sc-2789, which may also be suitable for detection of IgM.
Answered by: Technical Support
Date published: 2019-04-20
what if the antibody is kept at room temperature for more than 2 days accidentally? will it still work?
Asked by: debby
Thank you for the question. We suggest storing this antibody at +4°C - 8°C in the fridge. If stored accidentally at room temp. for 2 days the product likely will not be harmed. We still suggest evaluating the antibody with a positive control and verify the obtained data.
Answered by: Technical Support Europe
Date published: 2018-01-31
How can CD40 (HM40-3): sc-20010 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?
Asked by: jerojero
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-20010 PE) or fluoroscein (sc-20010 FITC).
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