CD4 Antibody (5B4) recommended for detection of CD4 of mouse, rat and human origin by WB, IP and FCM

CD4 Antibody (5B4): sc-70670

CD4 Antibody (5B4) is rated 4.3 out of 5 by 3.
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Datasheets

    • CD4 Antibody (5B4) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
    • raised against CD4+ transfectant followed by CEM cells
    • recommended for detection of CD4 of mouse, rat and human origin by WB, IP and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See CD4 (MT310): sc-19641 for CD4 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

What is the recommended starting dilution for Western blot for CD4 (5B4): sc-70670 monoclonal antibody?

Asked by: Trav11
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-70670.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from CD4 FITC ,Very good to use I use CD4 FITC to stain T cells to pick out CD4+ T cells. Very good to use
Date published: 2016-11-20
Rated 4 out of 5 by from Strong band in WB in HL Strong band in WB in HL-60 whole cell lysate. -SCBT QC
Date published: 2015-02-05
Rated 4 out of 5 by from Good publishable data in flow cytometry in Good publishable data in flow cytometry in human peripheral blood mononuclear cells (PBMC) samples. -SCBT Publication Review
Date published: 2014-03-24
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