CD35 Antibody (To5): sc-20055

CD35 Antibody (To5) is a mouse monoclonal IgG1 kappa light chain antibody that detects CD35 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). CD35 (To5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD35, also known as complement receptor 1 (CR1), plays a crucial role in the immune system by serving as a receptor for complement components C3b and C4b, which are essential for the opsonization and subsequent phagocytosis of pathogens by neutrophils and monocytes. This function is vital for the clearance of immune complexes and pathogens, thereby preventing excessive inflammation and tissue damage. CD35 is predominantly expressed on granulocytes, monocytes, B cells, certain natural killer cells, and erythrocytes, highlighting CD35′s importance in both innate and adaptive immunity. Notably, alterations in CD35 expression and function have been implicated in systemic lupus erythematosus (SLE), a chronic autoimmune disease characterized by the production of a wide array of autoantibodies and immune complex accumulation. In SLE patients, there is an observed increase in the proteolytic cleavage of leukocyte surface CD35, which may contribute to the dysregulation of immune responses. Sequence analysis indicates that Crry may represent the mouse genetic homolog of human CD35 antigen encoded by the CR1 gene, further emphasizing CD35′s relevance in both human and murine models of immune function.

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CD35 Antibody (To5) References:

1. Complement receptor type 1 (CD35) mediates inhibitory signals in human B lymphocytes.  |  Józsi, M., et al. 2002. J Immunol. 168: 2782-8. PMID: 11884446
2. Sites within the complement C3b/C4b receptor important for the specificity of ligand binding.  |  Krych, M., et al. 1991. Proc Natl Acad Sci U S A. 88: 4353-7. PMID: 1827918
3. Mapping of the C3b-binding site of CR1 and construction of a (CR1)2-F(ab')2 chimeric complement inhibitor.  |  Kalli, KR., et al. 1991. J Exp Med. 174: 1451-60. PMID: 1836011
4. Complement receptor type 1 (C3b/C4b receptor; CD35) and complement receptor type 2 (C3d/Epstein-Barr virus receptor; CD21).  |  Fearon, DT. and Ahearn, JM. 1990. Curr Top Microbiol Immunol. 153: 83-98. PMID: 2153507
5. Structure and function of the complement receptors, CR1 (CD35) and CR2 (CD21).  |  Ahearn, JM. and Fearon, DT. 1989. Adv Immunol. 46: 183-219. PMID: 2551147
6. Structure of the human CR1 gene. Molecular basis of the structural and quantitative polymorphisms and identification of a new CR1-like allele.  |  Wong, WW., et al. 1989. J Exp Med. 169: 847-63. PMID: 2564414
7. Brief Report: HIV-1 Infection Impairs CD16 and CD35 Mediated Opsonophagocytosis of Mycobacterium tuberculosis by Human Neutrophils.  |  Bangani, N., et al. 2016. J Acquir Immune Defic Syndr. 73: 263-267. PMID: 27258232
8. Human C3b/C4b receptor (CR1). Demonstration of long homologous repeating domains that are composed of the short consensus repeats characteristics of C3/C4 binding proteins.  |  Klickstein, LB., et al. 1987. J Exp Med. 165: 1095-112. PMID: 2951479
9. Identification of distinct C3b and C4b recognition sites in the human C3b/C4b receptor (CR1, CD35) by deletion mutagenesis.  |  Klickstein, LB., et al. 1988. J Exp Med. 168: 1699-717. PMID: 2972794
10. Combining MAP-1:CD35 or MAP-1:CD55 fusion proteins with pattern-recognition molecules as novel targeted modulators of the complement cascade.  |  Pérez-Alós, L., et al. 2019. FASEB J. 33: 12723-12734. PMID: 31469600
11. Expression levels of complement regulatory proteins (CD35, CD55 and CD59) on peripheral blood cells of patients with chronic kidney disease.  |  Eldewi, DM., et al. 2019. Int J Gen Med. 12: 343-351. PMID: 31571973
12. Distribution of C3-step regulatory proteins of the complement system, CD35 (CR1), CD46 (MCP), and CD55 (DAF), in hematological malignancies.  |  Seya, T., et al. 1994. Leuk Lymphoma. 12: 395-400. PMID: 7514063

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD35 Antibody (To5) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-CD35 Antibody (To5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD35 Antibody (To5) Specifications

Alternate Product Name: CD35

Product Clone Name: To5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: CD35

Epitope Species: human

Product Reactivity/Detection: CD35

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for CD35 Antibody (To5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin