CD35 Antibody (E-11): sc-7308

CD35 Antibody (E-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects CD35 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). CD35 (E-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD35, also known as complement receptor 1 (CR1), plays a crucial role in the immune response by serving as a receptor for complement components C3b and C4b, facilitating the phagocytosis of opsonized particles by neutrophils and monocytes. This function is vital for the clearance of pathogens and immune complexes, thereby maintaining homeostasis and preventing excessive inflammation. CD35 is predominantly expressed on granulocytes, monocytes, B cells, certain natural killer cells, and erythrocytes, highlighting its importance in both innate and adaptive immunity. Dysregulation of CD35 has been implicated in systemic lupus erythematosus (SLE), a chronic autoimmune condition characterized by the production of a wide array of autoantibodies and immune complex overload, which can lead to tissue damage. Notably, patients with SLE exhibit increased proteolytic cleavage of CD35 on leukocyte surfaces, which may contribute to the pathogenesis of the disease. Sequence analysis indicates that Crry may represent the mouse genetic homolog of human CD35 antigen encoded by the CR1 gene, further underscoring the relevance of CD35 in immunological research and therapeutic applications.

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CD35 Antibody (E-11) References:

1. Splenic CD19-CD35+B220+ cells function as an inducer of follicular dendritic cell network formation.  |  Murakami, T., et al. 2007. Blood. 110: 1215-24. PMID: 17519390
2. Implication of CD21, CD35, and CD55 in the pathogenesis of age-related macular degeneration.  |  Haas, P., et al. 2011. Am J Ophthalmol. 152: 396-399.e1. PMID: 21669404
3. Activation of CD35 and CD55 in HIV associated normal and pre-eclamptic pregnant women.  |  Khan, R., et al. 2016. Eur J Obstet Gynecol Reprod Biol. 204: 51-6. PMID: 27521598
4. Protein-engineered molecules carrying GAD65 epitopes and targeting CD35 selectively down-modulate disease-associated human B lymphocytes.  |  Manoylov, IK., et al. 2019. Clin Exp Immunol. 197: 329-340. PMID: 31009057
5. CD89/CD35 Expression Ratio in Salivary Neutrophil as an Early Detection Marker for Severe Early Childhood Caries.  |  Luthfi, M., et al. 2020. Eur J Dent. 14: 386-392. PMID: 32645730
6. Complement Receptor 1 (CR1)/ CD35+ Expression Analysis of Salivary Neutrophils on Streptococcus mutans Phagocytosis.  |  Luthfi, M., et al. 2019. Contemp Clin Dent. 10: 627-630. PMID: 32792821
7. Acquired decrease of the C3b/C4b receptor (CR1, CD35) and increased C4d deposits on erythrocytes from ICU COVID-19 patients.  |  Kisserli, A., et al. 2021. Immunobiology. 226: 152093. PMID: 34022670
8. The potential differential diagnosis value and clinical significance of CD35 expression in B-chronic lymphoproliferative disorders.  |  Shi, Y., et al. 2021. Ann Transl Med. 9: 1166. PMID: 34430607
9. Genetic variant in complement receptor 1 (CR1, CD35) is associated with a cluster of severe fatal COVID-19 in a family.  |  Danial-Farran, N., et al. 2023. J Infect. 87: 336-338. PMID: 36924837
10. Cross-linking CD21/CD35 or CD19 increases both B7-1 and B7-2 expression on murine splenic B cells.  |  Kozono, Y., et al. 1998. J Immunol. 160: 1565-72. PMID: 9469411

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD35 Antibody (E-11) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-CD35 Antibody (E-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD35 Antibody (E-11) Specifications

Alternate Product Name: Complement Receptor

Product Clone Name: E-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: CD35

Epitope Species: human

Product Reactivity/Detection: CD35

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for CD35 Antibody (E-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred CD35 antibody is CD35 Antibody (To5): sc-20055.