CD34 Antibody (ICO115): sc-7324

CD34 Antibody (ICO115) is a mouse monoclonal IgG1 kappa light chain antibody that detects CD34 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). CD34 (ICO115) antibody is available in both non-conjugated and various conjugated forms, including agarose, phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD34 is a heavily glycosylated transmembrane glycoprotein that plays a crucial role in identifying and isolating hematopoietic stem and progenitor cells, as CD34 is predominantly expressed on their surface. This expression is particularly significant because CD34 serves as a marker for the most primitive stem cells, which are essential for maintaining hematopoiesis and tissue regeneration. As these stem cells differentiate into lineage-committed progenitors, CD34 expression gradually diminishes, highlighting CD34′s importance in stem cell biology and potential therapeutic applications. CD34 is found on small-vessel endothelial cells and certain cells in fetal and adult nervous tissue, further underscoring CD34′s diverse roles in various biological contexts. The cytoplasmic domain of CD34 contains consensus sites for phosphorylation by activated protein kinase C and other kinases, which may influence signaling pathways critical for cell adhesion and migration.

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CD34 Antibody (ICO115) References:

1. Comparison of CD34 and monocyte-derived dendritic cells from mobilized peripheral blood from cancer patients.  |  Syme, R., et al. 2005. Stem Cells. 23: 74-81. PMID: 15625124
2. CD34 and CD43 inhibit mast cell adhesion and are required for optimal mast cell reconstitution.  |  Drew, E., et al. 2005. Immunity. 22: 43-57. PMID: 15664158
3. Tumor skewing of CD34+ cell differentiation from a dendritic cell pathway into endothelial cells.  |  Young, MR. and Cigal, M. 2006. Cancer Immunol Immunother. 55: 558-68. PMID: 16025265
4. Characterization of murine CD34, a marker for hematopoietic progenitor and stem cells.  |  Krause, DS., et al. 1994. Blood. 84: 691-701. PMID: 7519070
5. CD34+ positive haemopoietic cells: biology and clinical applications.  |  Holyoake, TL. and Alcorn, MJ. 1994. Blood Rev. 8: 113-24. PMID: 7524841
6. Expression of CD34 in endothelial cells, hematopoietic progenitors and nervous cells in fetal and adult mouse tissues.  |  Lin, G., et al. 1995. Eur J Immunol. 25: 1508-16. PMID: 7542195
7. Selectin-carbohydrate interactions and the initiation of the inflammatory response.  |  Lasky, LA. 1995. Annu Rev Biochem. 64: 113-39. PMID: 7574477
8. Cell adhesion receptors and early mammalian heart development: an overview.  |  Buck, CA., et al. 1993. C R Acad Sci III. 316: 838-59. PMID: 8076214
9. Platelet endothelial cell adhesion molecule (CD31).  |  DeLisser, HM., et al. 1993. Curr Top Microbiol Immunol. 184: 37-45. PMID: 8313722

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD34 Antibody (ICO115) has 234 citations in a variety of scientific publications.

Primary antibodies like Anti-CD34 Antibody (ICO115) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD34 Antibody (ICO115) Specifications

Alternate Product Name: CD34 stem cell marker

Product Clone Name: ICO115

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: CD34

Epitope Species: human

Product Reactivity/Detection: CD34

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 234 citations in various scientific publications

Available Conjugates/Forms for CD34 Antibody (ICO115): AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, PE: Phycoerythrin