CD3-γ Antibody (H-7): sc-393271

CD3-γ Antibody (H-7) is a mouse monoclonal IgG3 kappa light chain antibody that detects CD3-gamma protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-CD3-gamma antibody (H-7) is available as the non-conjugated form. CD3-gamma protein plays a crucial role in the immune response by forming part of the T cell receptor (TCR) complex, which is essential for T cell activation and signaling. CD3-gamma protein is located on the surface of T cells, where CD3-gamma protein associates with TCR to facilitate recognition of foreign antigens. Upon antigen binding, TCR-CD3 complex transmits signals that lead to T cell activation, proliferation, and differentiation, which are vital for mounting an effective immune response. CD3 complex is composed of five invariant polypeptide chains that form three distinct dimers, including gamma-epsilon heterodimer, which is critical for stability and function of TCR. Presence of immunoreceptor tyrosine-based activation motifs (ITAMs) within CD3 chains is essential for initiating downstream signaling cascades, making CD3-gamma protein a key player in adaptive immunity. Understanding CD3-gamma′s role in T cell signaling not only provides insights into immune system function but also has implications for therapeutic strategies targeting T cell responses in various diseases, including cancer and autoimmune disorders.

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CD3-γ Antibody (H-7) References:

1. TCR dynamics in human mature T lymphocytes lacking CD3 gamma.  |  Torres, PS., et al. 2003. J Immunol. 170: 5947-55. PMID: 12794121
2. CD3 and CD2 antigen-mediated CD3 gamma-chain phosphorylation in permeabilized human T cells. Regulation by cytosolic phosphatases.  |  Alexander, DR., et al. 1992. Biochem J. 288 (Pt 1): 69-77. PMID: 1359883
3. Structure, assembly and intracellular transport of the T cell receptor for antigen.  |  Exley, M., et al. 1991. Semin Immunol. 3: 283-97. PMID: 1686832
4. Signal transduction by the T cell antigen receptor.  |  Weiss, A., et al. 1991. Semin Immunol. 3: 313-24. PMID: 1839225
5. Human T cell leukemia virus type I prevents cell surface expression of the T cell receptor through down-regulation of the CD3-gamma, -delta, -epsilon, and -zeta genes.  |  de Waal Malefyt, R., et al. 1990. J Immunol. 145: 2297-303. PMID: 2144550
6. Signal transduction. Zapping tandem SH2 domains.  |  Weiss, A. 1995. Nature. 377: 17-8. PMID: 7659151
7. Molecular basis for interaction of the protein tyrosine kinase ZAP-70 with the T-cell receptor.  |  Hatada, MH., et al. 1995. Nature. 377: 32-8. PMID: 7659156
8. Binding of ZAP-70 to phosphorylated T-cell receptor zeta and eta enhances its autophosphorylation and generates specific binding sites for SH2 domain-containing proteins.  |  Neumeister, EN., et al. 1995. Mol Cell Biol. 15: 3171-8. PMID: 7760813
9. Different cytoplasmic structure of the CD3 zeta family dimer modulates the activation signal and function of T cells.  |  Aoe, T., et al. 1994. Int Immunol. 6: 1671-9. PMID: 7865460
10. The role of protein tyrosine kinases and protein tyrosine phosphatases in T cell antigen receptor signal transduction.  |  Chan, AC., et al. 1994. Annu Rev Immunol. 12: 555-92. PMID: 8011291
11. Targeted disruption of the CD3 eta locus causes high lethality in mice: modulation of Oct-1 transcription on the opposite strand.  |  Ohno, H., et al. 1994. EMBO J. 13: 1157-65. PMID: 8131747
12. Signaling through a CD3 gamma-deficient TCR/CD3 complex in immortalized mature CD4+ and CD8+ T lymphocytes.  |  Pacheco-Castro, A., et al. 1998. J Immunol. 161: 3152-60. PMID: 9743383

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD3-γ Antibody (H-7) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-CD3-γ Antibody (H-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD3-γ Antibody (H-7) Specifications

Alternate Product Name: CD3 gamma

Product Clone Name: H-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 151-180

Epitope Target: CD3-γ

Epitope Species: human

Product Reactivity/Detection: CD3-γ

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for CD3-γ Antibody (H-7): P: Blocking Peptide

The preferred CD3-δ antibody is CD3-δ Antibody (F-1): sc-137137.