CD3 Antibody (RM0027-3B19): sc-101442

CD3 Antibody (RM0027-3B19) is a rat monoclonal IgG2b antibody designed to target the CD3 molecule, also known as the T cell receptor-CD3 complex or cluster of differentiation 3. CD3 monoclonal antibody (RM0027-3B19) is raised against CD3 positive T cells of mouse origin and exhibits strong reactivity with mouse CD3 in applications such as immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). The CD3 complex is integral to T cell activation, consisting of five invariant polypeptide chains that form three distinct dimers: γε, δε, and ζζ or ζη. A critical aspect of CD3 function involves post-translational modifications, particularly the phosphorylation of immunoreceptor tyrosine-based activation motifs (ITAMs) within the CD3γ, CD3δ, CD3ε, and CD3ζ chains. These phosphorylated ITAMs serve as docking sites for protein kinases like ZAP-70 and Syk, initiating downstream signaling pathways essential for T cell responses. The structural integrity and proper post-translational modification of the CD3 complex are crucial for its role in transducing extracellular antigen recognition into intracellular signals that activate T cells. Anti-CD3 antibody (RM0027-3B19) facilitates detailed studies of T cell receptor dynamics and immune signaling, making this reagent an invaluable tool for both basic research and clinical applications in immunology.

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD3 Antibody (RM0027-3B19) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-CD3 Antibody (RM0027-3B19) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD3 Antibody (RM0027-3B19) Specifications

Alternate Product Name: CD3

Product Clone Name: RM0027-3B19

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b

Amino Acids:

Epitope Target: CD3

Epitope Species: mouse

Product Reactivity/Detection: CD3

Species Reactivity/Detection: mouse

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 100 µg IgG_2b in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for CD3 Antibody (RM0027-3B19): N/A

The preferred CD3 antibody is CD3 Antibody (PC3/188A): sc-20047.