CD1D Antibody (G-12): sc-373858
- CD1D Antibody (G-12) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 115-151 within an internal region of CD1D of mouse origin
- Anti-CD1D Antibody (G-12) is recommended for detection of CD1D of mouse, rat and mink origin by WB, IP, IF and ELISA
- Anti-CD1D Antibody (G-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CD1D Antibody (G-12) for WB applications. These reagents are now offered in bundles with CD1D Antibody (G-12) (see ordering information below).
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CD1D Antibody (G-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects CD1D in mouse, rat, and mink samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CD1D (G-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The CD1 multigene family encodes five distinct forms of CD1 T cell surface glycoprotein in humans: CD1A, CD1B, CD1C, CD1D, and CD1E. CD1D plays a crucial role in the immune system by presenting lipid antigens to T lymphocytes, which is essential for immune cell activation. CD1D is structurally similar to major histocompatibility complex (MHC) class I molecules and associates with β-2-Microglobulin, expressing on cortical thymocytes, Langerhans cells, a subset of B cells, and certain dendritic cells. Adaptor protein complexes and CD1-associated chaperones regulate CD1D-restricted T cell trafficking and functional maturation. CD1D appears on human intestinal epithelial cells in two forms: a β-2-Microglobulin-independent nonglycosylated variant and a β-2-Microglobulin-dependent glycosylated form. The human CD1D gene, located on chromosome 1q23.1, encodes a 335 amino acid protein vital for normal T cell maturation, highlighting CD1D′s importance in maintaining immune homeostasis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CD1D Antibody (G-12) References:
- CD1d-mediated antigen presentation to natural killer T (NKT) cells. | Brutkiewicz, RR., et al. 2003. Crit Rev Immunol. 23: 403-19. PMID: 15030309
- Regulation of antitumour immunity by CD1d-restricted NKT cells. | Swann, J., et al. 2004. Immunol Cell Biol. 82: 323-31. PMID: 15186264
- Lipid and glycolipid antigens of CD1d-restricted natural killer T cells. | Venkataswamy, MM. and Porcelli, SA. 2010. Semin Immunol. 22: 68-78. PMID: 19945296
- Impact of CD1d deficiency on metabolism. | Kotas, ME., et al. 2011. PLoS One. 6: e25478. PMID: 21980475
- CD1d-lipid antigen recognition by the γδ TCR. | Uldrich, AP., et al. 2013. Nat Immunol. 14: 1137-45. PMID: 24076636
- Adipocyte-specific CD1d-deficiency mitigates diet-induced obesity and insulin resistance in mice. | Satoh, M., et al. 2016. Sci Rep. 6: 28473. PMID: 27329323
- CD1d-mediated activation of group 3 innate lymphoid cells drives IL-22 production. | Saez de Guinoa, J., et al. 2017. EMBO Rep. 18: 39-47. PMID: 27799287
- JNK2 modulates the CD1d-dependent and -independent activation of iNKT cells. | Liu, J., et al. 2019. Eur J Immunol. 49: 255-265. PMID: 30467836
- CD1d Modulates Colonic Inflammation in NOD2-/- Mice by Altering the Intestinal Microbial Composition Comprising Acetatifactor muris. | Lee, C., et al. 2019. J Crohns Colitis. 13: 1081-1091. PMID: 31094420
- Adipocyte CD1d Gene Transfer Induces T Cell Expansion and Adipocyte Inflammation in CD1d Knockout Mice. | Xiao, R., et al. 2022. J Immunol. 208: 2109-2121. PMID: 35418470
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD1D Antibody (G-12) | sc-373858 | 200 µg/ml | $322.00 | |||
CD1D Antibody (G-12): m-IgG Fc BP-HRP Bundle | sc-529590 | 200 µg Ab; 10 µg BP | $361.00 | |||
CD1D Antibody (G-12): m-IgGκ BP-HRP Bundle | sc-522532 | 200 µg Ab, 40 µg BP | $361.00 | |||
CD1D Antibody (G-12) AC | sc-373858 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CD1D Antibody (G-12) HRP | sc-373858 HRP | 200 µg/ml | $322.00 | |||
CD1D Antibody (G-12) FITC | sc-373858 FITC | 200 µg/ml | $336.00 | |||
CD1D Antibody (G-12) PE | sc-373858 PE | 200 µg/ml | $349.00 | |||
CD1D Antibody (G-12) Alexa Fluor® 488 | sc-373858 AF488 | 200 µg/ml | $364.00 | |||
CD1D Antibody (G-12) Alexa Fluor® 546 | sc-373858 AF546 | 200 µg/ml | $364.00 | |||
CD1D Antibody (G-12) Alexa Fluor® 594 | sc-373858 AF594 | 200 µg/ml | $364.00 | |||
CD1D Antibody (G-12) Alexa Fluor® 647 | sc-373858 AF647 | 200 µg/ml | $364.00 | |||
CD1D Antibody (G-12) Alexa Fluor® 680 | sc-373858 AF680 | 200 µg/ml | $364.00 | |||
CD1D Antibody (G-12) Alexa Fluor® 790 | sc-373858 AF790 | 200 µg/ml | $364.00 | |||
CD1D (G-12) Neutralizing Peptide | sc-373858 P | 100 µg/0.5 ml | $69.00 |