CD151 Antibody (H-8): sc-271216

CD151 Antibody (H-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects CD151 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). CD151 (H-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD151 plays a crucial role in cell adhesion and integrin transport via vesicles, which is essential for maintaining tissue structural integrity and facilitating cellular communication. Located primarily in the plasma membrane, CD151 is a member of the tetraspanin superfamily and is involved in forming specialized junctional complexes known as hemidesmosomes, which anchor cells to the extracellular matrix. The human CD151 gene is mapped to chromosome 11p15.5 and encodes a 253-amino acid protein that interacts with various integrin chains, including β1, β3, β4, α2, α3, α5, and α6 integrins, thereby influencing cell motility, invasion, and metastasis in cancer cells through focal adhesion kinase-dependent pathways. RNA transcript of CD151 can be detected in several cell types, including MO7e cells, bone marrow stromal cells, C11 endothelial cells, and human umbilical vein endothelial cells, while absent in brain tissue and certain lymphoblastoid cell lines. The specific region of CD151, Leu149-Glu213, serves as the binding interface for integrins, highlighting its importance in cellular interactions and signaling pathways.

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CD151 Antibody (H-8) References:

1. PETA-3/CD151, a member of the transmembrane 4 superfamily, is localised to the plasma membrane and endocytic system of endothelial cells, associates with multiple integrins and modulates cell function.  |  Sincock, PM., et al. 1999. J Cell Sci. 112 (Pt 6): 833-44. PMID: 10036233
2. The tetraspan molecule CD151, a novel constituent of hemidesmosomes, associates with the integrin alpha6beta4 and may regulate the spatial organization of hemidesmosomes.  |  Sterk, LM., et al. 2000. J Cell Biol. 149: 969-82. PMID: 10811835
3. Differential tissue expression of epitopes of the tetraspanin CD151 recognised by monoclonal antibodies.  |  Geary, SM., et al. 2001. Tissue Antigens. 58: 141-53. PMID: 11703821
4. CD151.  |  Ashman, LK. 2002. J Biol Regul Homeost Agents. 16: 223-6. PMID: 12456024
5. Tetraspanin CD151 as a target for antibody-based cancer immunotherapy.  |  Haeuw, JF., et al. 2011. Biochem Soc Trans. 39: 553-8. PMID: 21428938
6. CD151 in cancer progression and metastasis: a complex scenario.  |  Sadej, R., et al. 2014. Lab Invest. 94: 41-51. PMID: 24247563
7. CD151-A Striking Marker for Cancer Therapy.  |  Kumari, S., et al. 2015. Biomark Cancer. 7: 7-11. PMID: 25861224
8. CD151 Expression Is Associated with a Hyperproliferative T Cell Phenotype.  |  Seu, L., et al. 2017. J Immunol. 199: 3336-3347. PMID: 28954890
9. CD151 and prostate cancer progression: A review of current literature.  |  Kang, Z., et al. 2023. Asia Pac J Clin Oncol. 19: 434-438. PMID: 36448565
10. CD151 Maintains Endolysosomal Protein Quality to Inhibit Vascular Inflammation.  |  Chen, J., et al. 2024. Circ Res. 134: 1330-1347. PMID: 38557119
11. Molecular cloning of cDNA encoding a novel platelet-endothelial cell tetra-span antigen, PETA-3.  |  Fitter, S., et al. 1995. Blood. 86: 1348-55. PMID: 7632941
12. SFA-1, a novel cellular gene induced by human T-cell leukemia virus type 1, is a member of the transmembrane 4 superfamily.  |  Hasegawa, H., et al. 1996. J Virol. 70: 3258-63. PMID: 8627808

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD151 Antibody (H-8) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-CD151 Antibody (H-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD151 Antibody (H-8) Specifications

Alternate Product Name: Tetraspanin 24

Product Clone Name: H-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 101-180

Epitope Target: CD151

Epitope Species: human

Product Reactivity/Detection: CD151

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for CD151 Antibody (H-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin