Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD14 CRISPR/Cas9 KO Plasmid (h): sc-400344
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD14 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CD14 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD14 Antibody (5A3B11B5): sc-58951
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD14 CRISPR/Cas9 KO Plasmid (h) | sc-400344 | 20 µg | $397.00 |
Overview
CD14 is a glycosylphosphatidylinositol-anchored pattern-recognition co-receptor that binds bacterial lipopolysaccharide in conjunction with LBP and transfers ligands to the TLR4–MD-2 complex to initiate innate immune signaling. It functions in monocytes, macrophages, and dendritic cells to amplify MyD88- and TRIF-dependent pathways, driving NF-κB and IRF activation, cytokine production, and inflammatory gene expression. Beyond endotoxin sensing, CD14 contributes to recognition of diverse pathogen- and damage-associated molecular patterns and modulates phagocytosis and antigen presentation. Dysregulated CD14 signaling has been linked to aberrant inflammatory responses implicated in sepsis biology, chronic inflammatory disorders, and tumor-associated myeloid phenotypes relevant to immune regulation.
CD14 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CD14 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CD14 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CD14 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CD14 protein expression.
This CRISPR knockout system enables efficient generation of CD14-deficient cell models for investigation of CD14 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CD14 exon(s) critical for CD14 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CD14 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CD14 CRISPR/Cas9 KO Plasmid (h) and CD14 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CD14 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CD14 HDR Plasmid (h) and CD14 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CD14 homology arms to support homology-directed repair at defined CD14 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.