Date published: 2025-10-7

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CCDC33 Antibody (F-3): sc-377074

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Datasheets
  • CCDC33 Antibody (F-3) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
  • raised against amino acids 659-849 mapping near the C-terminus of CCDC33 of human origin
  • recommended for detection of CCDC33 of human origin by WB, IP, IF, IHC(P) and ELISA
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for CCDC33 Antibody (F-3) for WB and IHC(P) applications. These reagents are now offered in bundles with CCDC33 Antibody (F-3) (see ordering information below).

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    CCDC33 Antibody (F-3) is a mouse monoclonal IgG1 antibody that detects CCDC33 in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). CCDC33, also known as CT61 (cancer/testis antigen 61), is a 958 amino acid protein predominantly expressed in male germ cells and plays a crucial role in spermatogenesis. CCDC33 is characterized by the presence of a coiled-coil domain, which is essential for mediating protein-protein interactions and is involved in various biological processes such as gene expression regulation and membrane fusion. The structural integrity provided by the coiled-coil motif is vital for proper CCDC33 functioning, as this feature facilitates the assembly of protein complexes necessary for sperm cell development. CCDC33 exists in at least four alternatively spliced isoforms, which may contribute to functional diversity and regulatory mechanisms within the germline. The gene encoding CCDC33 is located on human chromosome 15, a region associated with several genetic disorders, highlighting CCDC33′s importance in both normal physiology and potential pathologies.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    CCDC33 Antibody (F-3)

    sc-377074
    200 µg/ml
    $316.00

    CCDC33 Antibody (F-3): m-IgG Fc BP-HRP Bundle

    sc-540693
    200 µg Ab; 10 µg BP
    $354.00

    CCDC33 Antibody (F-3): m-IgG1 BP-HRP Bundle

    sc-542302
    200 µg Ab; 20 µg BP
    $354.00

    Can CCDC33 (F-3): sc-377074 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

    Asked by: Germaine
    Thank you for your question. Yes, CCDC33 (F-3): sc-377074 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
    Answered by: Technical Support
    Date published: 2017-03-01
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    Rated 5 out of 5 by from Exceptional immunoperoxidase cytoplasmicExceptional immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human prostate tissue. -SCBT QC
    Date published: 2015-03-22
    Rated 5 out of 5 by from Good Western Blot data of CCDC33 expression in nonGood Western Blot data of CCDC33 expression in non-transfected and human CCDC33 transfected 293T whole cell lysates. -SCBT QC
    Date published: 2014-11-05
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    CCDC33 Antibody (F-3) is rated 5.0 out of 5 by 2.
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