CBP80 Antibody (E-7): sc-271304

CBP80 Antibody (E-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects CBP80 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-CBP80 antibody (E-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CBP80 plays a crucial role in mRNA metabolism, particularly in the context of the cap-binding complex (CBC), which is essential for pre-mRNA splicing, 3′ end formation, and the export of U snRNA. CBC is composed of two cap-binding proteins, CBP20 and CBP80, which work together to enhance the functions of the m(7)G cap added to the majority of eukaryotic mRNAs. This cap structure is vital for the stability and translation of mRNA, as CBP80 protects the transcript from degradation and facilitates the recruitment of the translation machinery. CBP80, which consists of three domains each containing a MIF4G domain, interacts with CBP20, which has an RNAP fold, to form a stable complex that is critical for the initiation of translation and the regulation of nonsense-mediated decay (NMD). During the pioneer round of translation, CBP80-bound mRNA is processed before being replaced by eIF4E and other proteins, ensuring that only properly processed mRNAs are translated. This intricate regulation underscores CBP80′s importance in gene expression and cellular function, making CBP80 (E-7) monoclonal antibody an invaluable tool for researchers studying mRNA metabolism and related pathways.

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CBP80 Antibody (E-7) References:

1. The role of nuclear cap binding protein Cbc1p of yeast in mRNA termination and degradation.  |  Das, B., et al. 2000. Mol Cell Biol. 20: 2827-38. PMID: 10733586
2. Interaction of eukaryotic translation initiation factor 4G with the nuclear cap-binding complex provides a link between nuclear and cytoplasmic functions of the m(7) guanosine cap.  |  McKendrick, L., et al. 2001. Mol Cell Biol. 21: 3632-41. PMID: 11340157
3. Crystal structure of the human nuclear cap binding complex.  |  Mazza, C., et al. 2001. Mol Cell. 8: 383-96. PMID: 11545740
4. Evidence for a pioneer round of mRNA translation: mRNAs subject to nonsense-mediated decay in mammalian cells are bound by CBP80 and CBP20.  |  Ishigaki, Y., et al. 2001. Cell. 106: 607-17. PMID: 11551508
5. A cap-binding protein complex mediating U snRNA export.  |  Izaurralde, E., et al. 1995. Nature. 376: 709-12. PMID: 7651522
6. A nuclear cap binding protein complex involved in pre-mRNA splicing.  |  Izaurralde, E., et al. 1994. Cell. 78: 657-68. PMID: 8069914

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CBP80 Antibody (E-7) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-CBP80 Antibody (E-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CBP80 Antibody (E-7) Specifications

Alternate Product Name: CBP80

Product Clone Name: E-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 21-320

Epitope Target: CBP80

Epitope Species: human

Product Reactivity/Detection: CBP80

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for CBP80 Antibody (E-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent